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Lactobacillus plantarum and its extracellular polysaccharide

A technology of Lactobacillus plantarum and extracellular polysaccharide, applied in the field of microorganisms, can solve the problems of limited secretion amount and poor heavy metal adsorption effect, and achieve the effect of obvious adsorption effect.

Pending Publication Date: 2019-08-06
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing exopolysaccharide secretion of lactic acid bacteria is very limited, resulting in poor adsorption effect of heavy metals by lactic acid bacteria. Therefore, it is necessary to develop a biosorbent with good adsorption effect and sustainable development of heavy metals.

Method used

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  • Lactobacillus plantarum and its extracellular polysaccharide
  • Lactobacillus plantarum and its extracellular polysaccharide
  • Lactobacillus plantarum and its extracellular polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Lactobacillus plantarum 18-2 tolerance heavy metal test

[0032] Lactobacillus plantarum 18-2 was inoculated in MRS medium with different heavy metal ion concentration gradients and cultured for 24 hours, the growth of each strain of lactic acid bacteria was observed, and the highest ion concentration that could tolerate heavy metals was determined. The results show (see Table 2), Lactobacillus plantarum 18-2 can tolerate 200mg / L Cu 2+ , 1100mg / L Pb 2+ and 200mg / L Cd 2+ , has good application potential. In contrast, Lactobacillus plantarum 28-2 can tolerate 200mg / LCu 2+ , 600mg / L Pb 2+ and 900mg / L Cd 2+ . The adsorption effect of Lactobacillus plantarum 18-2 on the heavy metal lead was obvious.

[0033] Table 2 Effect of Lactobacillus plantarum on different concentrations of Pb 2+ The adsorption rate (unit: %)

[0034]

Embodiment 2

[0035] The EPS heavy metal lead adsorption test of embodiment 2Lactobacillus plantarum 18-2

[0036] Add 1 mL of 1 mg / mL Pb to 9 mL of EPS solutions with concentrations of 0 mg / mL, 1 mg / mL, 1.5 mg / mL, 2 mg / mL, 2.5 mg / mL and 3 mg / mL, respectively 2+ Mix the solution with a vortex oscillator and place it in a constant temperature water bath at 37°C for 4 hours. During this period, stir frequently to allow it to fully absorb. Then, centrifuge it with a centrifuge for 20 minutes at a speed of 4000 rpm, and then take the supernatant. Pb 2+ determination. It was found that when the 2-component EPS of Lactobacillus plantarum 18-2 was at a concentration of 1mg / mL and 1.5mg / ml, the 2+ Showed a high adsorption rate (see Table 3).

[0037] Table 3 Different concentrations of EPS to Pb 2+ The adsorption rate (unit: %)

[0038]

[0039]

[0040] Therefore, among the present invention, EPSA and EPSB are best to the adsorption effect of lead, when concentration is 1mg / ml concentra...

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Abstract

The invention discloses a Lactobacillus plantarum and an extracellular polysaccharide thereof. The strain is classified as Lactobacillus plantarum, and a strain number is Lactobacillus plantarum 18-2,which is preserved in the General Microbial Culture Collection Management Center of China, a preservation number is CGMCC No. 17566, and a preservation date is April 12, 2019. The Lactobacillus plantarum and its extracellular polysaccharide provided by the invention have obvious adsorption effect on heavy metal lead, on the one hand, the Lactobacillus plantarum 18-2 strain with heavy metal adsorption can be used as a starter in food production to prevent accumulation of heavy metal lead; and on the other hand, the EPS produced by Lactobacillus plantarum 18-2 strain has excellent adsorption ofheavy metal lead, and can be added as a heavy metal lead adsorption antidote alone or in combination to prevent the pollution and accumulation of heavy metal lead and ensure food safety.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a plant lactobacillus and its exopolysaccharide. Background technique [0002] With the advancement of industrialization, heavy metal pollution is becoming more and more serious. Heavy metal pollution not only causes great economic losses, but also endangers human health. Lead (Pb) is the most toxic heavy metal to the human body announced by the Food and Agriculture Organization of the United Nations (FAO) One of them is widely distributed in the environment, and is easily absorbed and enriched by organisms, and then acts on humans through the accumulation and amplification mechanism of the food chain, thereby endangering human health. [0003] Microbes in the environment cannot effectively degrade heavy metals and heavy metals cannot be decomposed by chemical reactions. Therefore, the toxicity of heavy metals cannot be eliminated directly. The toxic effects of heavy metal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20C12P19/04C12N1/20C12R1/25
CPCA23L5/273C12N1/20C12P19/04C12R2001/25C12N1/205
Inventor 旭日花
Owner INNER MONGOLIA UNIVERSITY