Biological polysaccharide with hair growth and hair loss prevention and its application
A kind of use, glucan technology, applied in the field of biotechnology and pharmaceutical preparations, can solve problems such as lack of hair growth drugs
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Embodiment 1
[0224] Example 1, acquisition and determination of β-glucan
[0225] This embodiment only takes the β-glucan obtained by fermentation of Schizophyllum as an example, but is not limited thereto.
[0226] 1. Obtaining the fermentation broth of Schizophyllum bacteria
[0227] The fermentation broth in the following examples was prepared by fermenting Schizophyllum species (Schizophyllum communeFr-1003, product catalogue: 38548 TM , purchased from the American Type Culture Collection Center [ATCC]), as follows:
[0228] 1. Activation of Schizophyllum strains: 200g / L of potato, 30g / L of glucose, 10g / L of sodium chloride and 20g / L of agar are made into a plate medium, and the strain of Schizophyllum is inoculated on the plate medium , cultured in a constant temperature incubator at 25°C for 7 days to obtain flat mycelium;
[0229] 2. Seed activation: put the liquid medium made of potato starch 100g / L, glucose 40g / L, yeast extract 2g / L, yeast extract powder 2g / L, and water into ...
Embodiment 2
[0281] Example 2 β-glucan promotes hair growth experiment (rat)
[0282] 1. Experimental method
[0283] 1. Take 8 Wistar rats as spares;
[0284] 2. Take 100g of rosin and paraffin in a small beaker, heat, melt and mix in a boiling water bath, and keep at 70°C to prevent solidification for later use;
[0285] 3. Weigh the body weight of each rat, and perform intraperitoneal injection anesthesia with trichloroacetaldehyde according to the dosage of 0.3% of the body weight;
[0286] 4. Select an area on the left and right sides of the back of the anesthetized rat, apply an appropriate amount of the rosin-paraffin wax mixture evenly, and after cooling and solidify for a while, peel it off against the direction of hair growth to expose the hairless rat back skin;
[0287] 5. The rats were divided into two groups after hair removal, and 1 mg / mL glycerol and 1 mg / mL Schizophyllum β-glucan (prepared according to the method of Example 1) were smeared on both sides of the back of ea...
Embodiment 3
[0291] Example 3 Beta-glucan optimizes the basic properties of AES
[0292] 1. Experimental method
[0293] 1. Take two tubes of 25 mL 10% AES aqueous solution, add 1 mg / mL Schizophyllum β-glucan (prepared according to the method in Example 1) in the experimental group, and add 1 mg / mL deionized water in the control group. The two tubes were mixed and shaken vigorously for 30 s, and the amount of foam produced was immediately observed.
[0294] 2. Take two tubes of 25 mL 10% AES aqueous solution, add 1 mg / mL Schizophyllum β-glucan (prepared according to the method in Example 1) in the experimental group, and add 1 mg / mL deionized water in the control group. The two tubes were mixed and shaken vigorously for 30 s, and the defoaming speed was observed immediately.
[0295] 3. Take two tubes of 25 mL 10% AES aqueous solution, add 1 mg / mL Schizophyllum β-glucan (prepared according to the method in Example 1) in the experimental group, and add 1 mg / mL deionized water in the contr...
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