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A squeeze device for increasing the number of extracellular vesicles produced by cells and its application

An extrusion device and cell technology, applied in the field of bioengineering, can solve problems such as increasing the production of extracellular vesicles, and achieve the effect of increasing production

Active Publication Date: 2020-08-11
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no mature technical way to increase the production of extracellular vesicles

Method used

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  • A squeeze device for increasing the number of extracellular vesicles produced by cells and its application
  • A squeeze device for increasing the number of extracellular vesicles produced by cells and its application
  • A squeeze device for increasing the number of extracellular vesicles produced by cells and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A squeeze device that increases the number of extracellular vesicles produced by cells, ref. figure 1 , including an extrusion device and an extrusion board; the extrusion device includes: a bracket 1, a steering gear 2, an arduino open source development board 3, a connecting wire 4 and a suction cup 5;

[0035] One end of the steering gear 2 is movably connected to the bracket 1, so that the steering gear 2 can move left, right, up and down relative to the bracket 1; the arduino open source development board 3 communicates with the steering gear 2 to control the steering gear The other end of the steering gear 2 is connected to one end of the connection line 4, the other end of the connection line 4 is connected to the suction cup 5, and the extrusion plate 6 is adsorbed on the suction cup and then the extrusion plate, The lower surface is perpendicular to the connection line 4; the surface of the extruded plate 6 is a porous structure of gallium nitride formed after ...

Embodiment 2

[0038] An extrusion device for increasing the number of extracellular vesicles produced by cells, the same as Example 1, the difference is that the preparation method of the porous structure of the gallium nitride component is: using the device and experimental method in the 201180007613.2 patent: using oxalic acid As an electrolyte, its concentration is 0.03M. n-type doped GaN (10 18 cm -3 ) and platinum wire constitute an anode and a cathode, which are respectively connected to a power source to form a circuit, the voltage is controlled at 30v, the etching rate is 200nm / min, and the etching time is 5min. After etching, use deionized water, methanol and pentane to wash in sequence and dry for use.

Embodiment 3

[0040] The extrusion device for increasing the number of extracellular vesicles produced by cells is the same as in Example 1, the difference is that it also includes a counterweight 7 (reference figure 2); The counterweight 7 is a polyethylene sheet with a circular hole in the middle, and its shape and specifications are consistent with the extruded plate. When in use, the counterweight 7 is placed above the extruded plate, and the round hole in the middle of the counterweight 7 is for the connecting wire 4 and the sucker 5 to pass through.

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Abstract

The invention belongs to the field of a bioengineering technology, and particularly relates to an extruding device for increasing the number of extracellular vesicles produced by cells and an application thereof. The extruding device comprises: a pressing device and a pressing plate; the pressing device comprises: a bracket, a steering gear, an arduino open-source development board, a connecting line and a suction cup; one end of the steering gear is movably connected to the bracket to enable the steering gear to move relative to the bracket; the arduino open-source development board is connected with the steering gear to control the steering gear to perform an action; the other end of the steering gear is connected to one end of a connecting line, and the other end of the connecting lineis connected with the suction cup; the surface of the pressing plate is a porous structure of a gallium nitride component; the surface of the pressing plate is the surface in contact with the cells requiring to be extruded. The extruding device can effectively increase the production of extracellular vesicles because the surface of the pressing plate is the porous structure of the gallium nitridecomponent formed after treatment.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to an extrusion device for increasing the number of extracellular vesicles produced by cells Background technique [0002] In the present invention, the information disclosed in the background art is only intended to increase the understanding of the general background of the present invention, and is not necessarily regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art known to those skilled in the art . [0003] Secretion of extracellular vesicles is a universal function of cells. With the in-depth study of extracellular vesicles in recent years, their functions and specific mechanisms, including intercellular communication, have been gradually elucidated. Extracellular vesicles have shown potential as targeted drugs due to their ability to be taken up by other cells and play a regulatory role. However, extracellular vesic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/04
CPCC12M35/04
Inventor 李刚张宇薛皓韩琳郭钦栋刘宏
Owner SHANDONG UNIV