A Molecular Marker Closely Linked to Wheat Ear Extraction qtl QPel.HN.6D and Its Application
A molecular marker and molecular marker-assisted technology, applied in the field of molecular biology, can solve the problems of limited and undiscovered genes, and achieve the effects of accurate and efficient detection, convenient and stable amplification
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Embodiment 1
[0063] Example 1 The localization of QTL QPel.HN.6D for wheat panicle extraction and the acquisition of its closely linked molecular marker HRM6
[0064] 1.1 The F of H461×Chuannong 16 (CN16) was selected as the test material 9 In the RIL population, CN16 was the cultivar with longer panicle extraction, and H461 was the cultivar with short panicle extraction. Using wheat H461 as the female parent and wheat CN16 as the male parent, hybrid F was obtained 1 , F 1 Generation of individual plants to obtain F by selfing 2 , using single-seed propagation method to obtain F containing 249 lines 9 To generate the RIL population, 188 lines were randomly selected to constitute the genetic mapping population.
[0065] 1.2 Extract the DNA of each line of the described genetic mapping population by CTAB method, use the wheat 90K SNP chip technology, take the DNA of parents H461 and CN16 as templates, carry out genotyping, and obtain the genotype data of the RIL population . The obtai...
Embodiment 2
[0075] Example 2 Application of molecular marker HRM6 closely linked with wheat ear extraction QTL QPel.HN.6D
[0076] 2.1 Obtaining the verification group
[0077] H461 is a wheat mutant carrying the QTL QPel.HN.6D for panicle extraction, and the panicle extraction is shorter, while the wheat variety Chuanmai 107 (CM107) has a longer panicle extraction. Using H461 as the female parent and CM107 as the male parent, the hybrid F was obtained 1 , F 1 Generation of individual plants to obtain F by selfing 2 , using single-seed propagation method to obtain F containing 188 strains 8 Generation RIL population.
[0078] 2.2 Molecular marker HRM6 for genotyping
[0079] (1) Take the F obtained in step 2.1 8 The genomic DNA of 188 strains of the RIL population was used as the template, and HRM6-F (SEQ ID NO. 2) and HRM6-R (SEQ ID NO. 3) were used as primers. type.
[0080] Fluorescence quantitative PCR amplification reaction system (10 μL): 5 μL of SsoFast EvaGreen supermix, 3...
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