A Molecular Marker Closely Linked to Wheat Ear Extraction qtl QPel.HN.6D and Its Application

A molecular marker and molecular marker-assisted technology, applied in the field of molecular biology, can solve the problems of limited and undiscovered genes, and achieve the effects of accurate and efficient detection, convenient and stable amplification

Active Publication Date: 2022-04-19
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current genetic research on wheat ear extraction is still limited, and there are still new genes to be discovered

Method used

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  • A Molecular Marker Closely Linked to Wheat Ear Extraction qtl QPel.HN.6D and Its Application
  • A Molecular Marker Closely Linked to Wheat Ear Extraction qtl QPel.HN.6D and Its Application
  • A Molecular Marker Closely Linked to Wheat Ear Extraction qtl QPel.HN.6D and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 The localization of QTL QPel.HN.6D for wheat panicle extraction and the acquisition of its closely linked molecular marker HRM6

[0064] 1.1 The F of H461×Chuannong 16 (CN16) was selected as the test material 9 In the RIL population, CN16 was the cultivar with longer panicle extraction, and H461 was the cultivar with short panicle extraction. Using wheat H461 as the female parent and wheat CN16 as the male parent, hybrid F was obtained 1 , F 1 Generation of individual plants to obtain F by selfing 2 , using single-seed propagation method to obtain F containing 249 lines 9 To generate the RIL population, 188 lines were randomly selected to constitute the genetic mapping population.

[0065] 1.2 Extract the DNA of each line of the described genetic mapping population by CTAB method, use the wheat 90K SNP chip technology, take the DNA of parents H461 and CN16 as templates, carry out genotyping, and obtain the genotype data of the RIL population . The obtai...

Embodiment 2

[0075] Example 2 Application of molecular marker HRM6 closely linked with wheat ear extraction QTL QPel.HN.6D

[0076] 2.1 Obtaining the verification group

[0077] H461 is a wheat mutant carrying the QTL QPel.HN.6D for panicle extraction, and the panicle extraction is shorter, while the wheat variety Chuanmai 107 (CM107) has a longer panicle extraction. Using H461 as the female parent and CM107 as the male parent, the hybrid F was obtained 1 , F 1 Generation of individual plants to obtain F by selfing 2 , using single-seed propagation method to obtain F containing 188 strains 8 Generation RIL population.

[0078] 2.2 Molecular marker HRM6 for genotyping

[0079] (1) Take the F obtained in step 2.1 8 The genomic DNA of 188 strains of the RIL population was used as the template, and HRM6-F (SEQ ID NO. 2) and HRM6-R (SEQ ID NO. 3) were used as primers. type.

[0080] Fluorescence quantitative PCR amplification reaction system (10 μL): 5 μL of SsoFast EvaGreen supermix, 3...

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Abstract

The invention relates to the technical field of molecular biology, in particular to a molecular marker closely linked to wheat ear extraction QTL QPel.HN.6D and its application. The present invention provides a molecular marker closely linked to wheat ear extraction QTL QPel.HN.6D, the nucleotide sequence of which is shown in SEQ ID NO.1; molecular marker HRM6 is located at the end of wheat ear extraction QTL QPel.HN.6D Within the confidence interval, the two co-localized on wheat chromosome 6D. The molecular marker can accurately track the wheat panicle withdrawal QTL QPel.HN.6D, predict the characteristics of wheat panicle withdrawal, facilitate molecular design and breeding, and enhance the accuracy of ear withdrawal prediction, so as to quickly screen out QTLs with reduced panicle withdrawal Wheat varieties or strains are used for breeding, which can greatly speed up the breeding process of high-yielding wheat varieties with ideal plant types.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a molecular marker closely linked to wheat ear extraction QTL QPel.HN.6D and its application. Background technique [0002] Common wheat (Triticum aestivum L.) is one of the most widely planted cereals. As an important part of global food security, it accounts for about 20% of the calories consumed by humans. Increasing the yield potential of crops is a major goal of crop improvement programs worldwide. In wheat, increasing harvest index, improving leaf morphology and reducing plant height are key goals for increasing wheat yield. Studies have shown that panicle exsertion length (PEL) has the potential to increase harvest index and yield (Liang Yunjuan, Gao Qinglu, Xue Xiang. Gray correlation analysis of yield per plant, harvest index and main agronomic traits in wheat[J]. Acta Biomathematica, 2013, 2:335-360). Different from the internode length of the ear neck (the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/158
Inventor 刘亚西易鑫陶阳林宇王智强石浩然武方琨李彩霞魏育明郑有良
Owner SICHUAN AGRI UNIV
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