Molecular marker closely linked with wheat heading degree QTL QPel.HN.6D and application thereof

A molecular marker and wheat technology, applied in the field of molecular biology, can solve the problems of undiscovered and limited genes, and achieve the effect of improving efficiency

Active Publication Date: 2019-08-09
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current genetic research on wheat ear extraction i

Method used

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  • Molecular marker closely linked with wheat heading degree QTL QPel.HN.6D and application thereof
  • Molecular marker closely linked with wheat heading degree QTL QPel.HN.6D and application thereof
  • Molecular marker closely linked with wheat heading degree QTL QPel.HN.6D and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Mapping of wheat ear extraction QTL QPel.HN.6D and acquisition of its closely linked molecular marker HRM6

[0064] 1.1 The test material is selected from the F of H461×Chuannong 16 (CN16) 9 In the RIL population, CN16 is a variety with a longer panicle withdrawal, and H461 is a variety with a shorter panicle withdrawal. Using wheat H461 as the female parent and wheat CN16 as the male parent, the hybrid F 1 , F 1 F 2 , using the single-seed method to obtain F 9 To generate the RIL population, 188 lines were randomly selected to form the genetic mapping population.

[0065] 1.2 Use the CTAB method to extract the DNA of each strain of the genetic mapping population, use the wheat 90K SNP chip technology, use the DNA of the parents H461 and CN16 as templates, and perform genotyping to obtain the genotype data of the RIL population . The obtained genotype data of the RIL population were imported into the mapping software JoinMap4.0 to find the optimal marke...

Embodiment 2

[0075] Example 2 Application of Molecular Marker HRM6 Closely Linked to Wheat Ear Extraction QTL QPel.HN.6D

[0076] 2.1 Acquisition of groups for verification

[0077] H461 is a wheat mutant carrying QTL QPel.HN.6D for panicle withdrawal. The panicle withdrawal is shorter, while the wheat variety Chuanmai 107 (CM107) has a longer panicle withdrawal. Using H461 as the female parent and CM107 as the male parent to cross, the hybrid F 1 , F 1 F 2 , using the single-seed method to obtain F 8 Generation RIL groups.

[0078] 2.2 Molecular marker HRM6 for genotyping

[0079] (1) F obtained from step 2.1 8 The genomic DNA of 188 strains of the generation RIL population was used as a template, and HRM6-F (SEQ ID NO.2) and HRM6-R (SEQ ID NO.3) were used as primers to perform amplification and HRM analysis based on a fluorescent quantitative PCR platform. type.

[0080] Fluorescent quantitative PCR amplification reaction system (10 μL): 5 μL of SsoFast EvaGreen supermixture, 300...

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Abstract

The invention relates to the technical field of molecular biology, in particular to a molecular marker closely linked with the wheat heading degree QTL QPel.HN.6D and application thereof. The nucleotide sequence of the molecular marker closely linked with the wheat heading degree QTL QPel.HN.6D is as shown in SEQ ID NO.1. The molecular marker HRM6 is in the confidence interval of the wheat headingdegree QTL QPel.HN.6D, and the molecular marker and the wheat heading degree QTL QPel.HN.6D are both positioned on a wheat 6D chromosome. The molecular marker can accurately track the wheat heading degree QTL QPel.HN.6D, predict the characteristics of the wheat heading degree, facilitate molecular design breeding, enhance the prediction accuracy of the heading degree so as to quickly screen out wheat varieties or strains capable of reducing the heading degree QTL for breeding, and greatly accelerate the breeding process of high-yield wheat varieties which are identical in plant type.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a molecular marker closely linked to wheat ear extraction QTL QPel.HN.6D and its application. Background technique [0002] Common wheat (Triticum aestivum L.) is one of the most widely planted cereals. As an important part of global food security, it accounts for about 20% of the calories consumed by humans. Increasing the yield potential of crops is a major goal of crop improvement programs worldwide. In wheat, increasing harvest index, improving leaf morphology and reducing plant height are key goals for increasing wheat yield. Studies have shown that panicle exsertion length (PEL) has the potential to increase harvest index and yield (Liang Yunjuan, Gao Qinglu, Xue Xiang. Gray correlation analysis of yield per plant, harvest index and main agronomic traits in wheat[J]. Acta Biomathematica, 2013, 2:335-360). Different from the internode length of the ear neck (the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/158
Inventor 刘亚西易鑫陶阳林宇王智强石浩然武方琨李彩霞魏育明郑有良
Owner SICHUAN AGRI UNIV
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