Molecular marker closely linked with wheat heading degree QTL QPel.HN.6D and application thereof
A molecular marker and wheat technology, applied in the field of molecular biology, can solve the problems of undiscovered and limited genes, and achieve the effect of improving efficiency
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Embodiment 1
[0063] Example 1 Mapping of wheat ear extraction QTL QPel.HN.6D and acquisition of its closely linked molecular marker HRM6
[0064] 1.1 The test material is selected from the F of H461×Chuannong 16 (CN16) 9 In the RIL population, CN16 is a variety with a longer panicle withdrawal, and H461 is a variety with a shorter panicle withdrawal. Using wheat H461 as the female parent and wheat CN16 as the male parent, the hybrid F 1 , F 1 F 2 , using the single-seed method to obtain F 9 To generate the RIL population, 188 lines were randomly selected to form the genetic mapping population.
[0065] 1.2 Use the CTAB method to extract the DNA of each strain of the genetic mapping population, use the wheat 90K SNP chip technology, use the DNA of the parents H461 and CN16 as templates, and perform genotyping to obtain the genotype data of the RIL population . The obtained genotype data of the RIL population were imported into the mapping software JoinMap4.0 to find the optimal marke...
Embodiment 2
[0075] Example 2 Application of Molecular Marker HRM6 Closely Linked to Wheat Ear Extraction QTL QPel.HN.6D
[0076] 2.1 Acquisition of groups for verification
[0077] H461 is a wheat mutant carrying QTL QPel.HN.6D for panicle withdrawal. The panicle withdrawal is shorter, while the wheat variety Chuanmai 107 (CM107) has a longer panicle withdrawal. Using H461 as the female parent and CM107 as the male parent to cross, the hybrid F 1 , F 1 F 2 , using the single-seed method to obtain F 8 Generation RIL groups.
[0078] 2.2 Molecular marker HRM6 for genotyping
[0079] (1) F obtained from step 2.1 8 The genomic DNA of 188 strains of the generation RIL population was used as a template, and HRM6-F (SEQ ID NO.2) and HRM6-R (SEQ ID NO.3) were used as primers to perform amplification and HRM analysis based on a fluorescent quantitative PCR platform. type.
[0080] Fluorescent quantitative PCR amplification reaction system (10 μL): 5 μL of SsoFast EvaGreen supermixture, 300...
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