LAMP primer combination for detecting 4 gram-negative bacteria in intraocular fluid and application thereof

A primer combination and primer set technology, applied in the biological field, can solve the problems of long PCR detection time, easy contamination, and application limitations
CN110129316AInactive Publication Date: 2019-08-16BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
Publication Date
2019-08-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an LAMP primer combination for detecting 4 gram-negative bacteria in intraocular fluid and an application thereof. The primer combination provided by the invention firstly is composed of 24 DNA molecules shown in a sequence 1 to 24. The primer combination can be applied for detecting whether to-be-tested bacteria are escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae or acinetobacter baumannii, and can be applied for detecting whether to-be-tested samples contain escherichia coli and / or pseudomonas aeruginosa and / or klebsiella pneumoniae and / or acinetobacter baumannii. The primer combination provided by the invention is used for detecting 4 gram-negative bacteria in the intraocular fluid, has high specificity and sensitivity, can realize simple, rapid and accurate detection, and has great promotion value.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a combination and application of LAMP primers for detecting four kinds of Gram-negative bacteria in intraocular fluid. Background technique

[0002] Bacteria are the main pathogenic bacteria of exogenous endophthalmitis, Escherichia coli (Escherichia coli), Pseudomonas aeruginosa (P. The main 4 Gram-negative bacteria that cause endophthalmitis. Escherichia coli was discovered by Escherich in 1885. For a long period of time, it has been regarded as a part of normal intestinal flora and considered to be non-pathogenic bacteria. It was not until the middle of the 20th century that some special serotypes were recognized. Escherichia coli is pathogenic to humans and animals, and is the most dominant and most abundant bacterium in the intestinal tract of humans and many animals. Pseudomonas aeruginosa, formerly known as Pseudomonas aeruginosa, is a common conditional pathogen...

Claims

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