Application of vitamin C in preparation of products for inhibiting human oral squamous cell carcinoma (OSCC)

A technology for oral squamous cell carcinoma and vitamins, which is applied in the fields of beverages, biomedicine and food, and achieves the effects of high clinical application reliability, less toxic and side effects, and convenient access.

Inactive Publication Date: 2019-08-27
SHANGHAI CHANGZHENG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is no literature report on the relationship between vitamin C and the inhibition of human oral squamous cell carcinoma

Method used

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  • Application of vitamin C in preparation of products for inhibiting human oral squamous cell carcinoma (OSCC)
  • Application of vitamin C in preparation of products for inhibiting human oral squamous cell carcinoma (OSCC)
  • Application of vitamin C in preparation of products for inhibiting human oral squamous cell carcinoma (OSCC)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Effect of vitamin C on the growth of human oral squamous cell carcinoma cells

[0029] 1.1 Experimental materials

[0030] Vitamin C was purchased from Shanghai Sangon Bioengineering Co., Ltd.

[0031] Human oral squamous cell carcinoma CAL27, SCC9, and SCC25 cell lines were purchased from American type culture collection (ATCC).

[0032] 1.2 Experimental method

[0033] 1.2.1 Colony formation inhibition experiment

[0034] Take the logarithmic growth phase cells of CAL27, SCC9, and SCC25 respectively, inoculate them in 6-well plates with 150 cells per well, and place them in 5% CO 2 cultured in a 37°C incubator. A no-drug control group and two drug-intervention groups were set up. The concentrations of vitamin C in the drug-intervention groups were 0.3 mg / ml and 0.6 mg / ml, respectively, and each group had 3 replicate wells.

[0035] After the cells adhered to the wall, the drug treatment was started for 24 hours, the complete medium was changed, and the...

Embodiment 2

[0051] Example 2. The Effect of Vitamin C on the Growth of Transplanted Tumors in Nude Mice

[0052] 2.1 Experimental materials

[0053] Vitamin C was purchased from Shanghai Sangon Bioengineering Co., Ltd.

[0054] 4-week-old female thymus-deficient nude mice were purchased from Shanghai Jihui Experimental Animal Breeding Co., Ltd.

[0055] 2.2 Experimental method

[0056] Take human oral squamous cell carcinoma CAL27 cells in the logarithmic growth phase, digest with 0.25% trypsin and collect the cells, pipette the cells with PBS to make a cell suspension, adjust the suspension density to 8×10 7 pieces / ml. Inject 100ul of cell suspension subcutaneously into the left armpit of 16 nude mice, when the tumor size reaches 50mm 3 Start intraperitoneal drug injection. The nude mice were randomly divided into 4 groups, 4 mice in each group. Each group was injected with normal saline (100ul, 2 times / day), vitamin C (4g / kg, 2 times / day), cisplatin (4mg / kg, 3 times / week), vitamin...

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Abstract

The invention relates to the technical field of biological medicines, and provides application of vitamin C in preparation of products for inhibiting human oral squamous cell carcinoma (OSCC). The products for inhibiting the human OSCC comprise drugs for treating the human OSCC, and health care food or health care drink for preventing the human OSCC. It is verified by cell experiments and animal experiments that the vitamin C can inhibit the growth of human OSCC cells, increases the apoptosis of the human OSCC cells, inhibits the growth of nude mouse transplanted tumors at the same time, and is better in inhibitory effect on tumors in combination with cis-platinum, so that it is shown that the vitamin C has the effect of inhibiting the human OSCC. Therefore, the vitamin C is to become a novel drug for inhibiting the human OSCC, and new exploration is provided for the prevention or treatment of the human OSCC.

Description

technical field [0001] The invention relates to the technical fields of biomedicine, food and beverage, in particular to the new application of vitamin C, especially the application in the preparation of products for inhibiting human oral squamous cell carcinoma. Background technique [0002] Malignant tumors of the oral and maxillofacial region are common malignant tumors in the head and neck, and more than 90% of them are oral squamous cell carcinoma (OSCC). Oral squamous cell carcinoma is the eighth most malignant tumor in the world, and its main causes include smoking, drinking and viral infection. At present, the main treatment methods for oral squamous cell carcinoma are surgery, radiotherapy and chemotherapy, and immunotherapy. For patients with recurrence, metastasis and advanced stage, chemotherapy is still the main method. However, after comprehensive treatment, the 5-year survival rate of oral squamous cell carcinoma patients is still maintained at 40% to 50%. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/375A61P1/02A61P35/00A23L33/15A23L2/52
CPCA23L2/52A23V2002/00A61K31/375A23L33/15A61P1/02A61P35/00A23V2200/308
Inventor 周建军王国栋
Owner SHANGHAI CHANGZHENG HOSPITAL
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