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Seedling rapid propagation culture medium and seedling rapid propagation method of protocorm of oreorchis patens(Lindl.)Lindl.

A culture medium, protocorm technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long cycle and low multiplication coefficient

Active Publication Date: 2019-08-30
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Proliferation in the form of corm budding has a low multiplication coefficient and a long cycle, while the proliferation in the form of protocorms has a high multiplication coefficient, but there is one more step in which the protocorm differentiates into a corm.

Method used

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  • Seedling rapid propagation culture medium and seedling rapid propagation method of protocorm of oreorchis patens(Lindl.)Lindl.
  • Seedling rapid propagation culture medium and seedling rapid propagation method of protocorm of oreorchis patens(Lindl.)Lindl.
  • Seedling rapid propagation culture medium and seedling rapid propagation method of protocorm of oreorchis patens(Lindl.)Lindl.

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Experimental program
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Effect test

Embodiment 1

[0042] 1 Test materials and methods

[0043] 1.1 Test material

[0044] The protocorms produced by 1-year-old bulbs were used as explants, and the disinfection and cultivation methods of the bulbs were referred to related literature (Wang Pingping, Wang Yujiao, Chen Xuhui, et al. Research on in vitro culture conditions of mycorrhizal fungus of Shanlan[J]. North Horticulture, 2012 , (09):66-69.), the explants are protocorms with growth points, about 3*3*5mm in size.

[0045] 1.2 The effect of auxin on the proliferation and differentiation of protocorms

[0046] With MS+6-BA0.1mg / L+sucrose 30g / L+agar powder 4.5g / L as the basic medium, add 2,4-D, NAA, IBA, IAA each 0.1mg / L, 2.0mg / L, 4.0 mg / L, 6.0mg / L, 8.0mg / L, 10.0mg / L; the culture temperature is 23°C, the light-dark cycle is 14h / 10h, the light intensity is 1500lx, and the culture time is 55d. For explants, the statistical indicators are the protocorm branching elongation proliferation differentiation coefficient, protocorm diff...

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Abstract

The invention relates to the technical field of tissue culture, in particular to a seedling rapid propagation culture medium and seedling rapid propagation method of protocorm of oreorchis patens(Lindl.)Lindl.. The seedling rapid propagation culture medium is prepared from an elongation and multiplication culture medium which is an MS culture medium containing sucrose, agar powder, NAA and ZT, a differentiation culture medium which is an MS culture medium containing sucrose, agar powder, IAA and 6-BA and a rooting culture medium which is an MS culture medium containing sucrose, agar powder, IAA and 6-BA. Under the condition of tissue culture, and under the control of hormones, the growth rate of the protocorm is high, the branched elongation and multiplication coefficient reaches 8.67, andthe corm coefficient generated through protocorm differentiation reaches 5.14, that is to say, after one protocorm is subjected to subculture multiplication and differentiation culture, about 45 robust seedlings with complete roots, stems and leaves can be produced.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a seedling rapid propagation medium and a seedling rapid propagation method of protocorm spp. Background technique [0002] Oreorchis patens (Lindl.) Lindl. is a perennial shade herbaceous plant of Orchidaceae Oreorchis. It is widely distributed in Southwest my country, Central China, Northwest China and Northeast China. It also has a small amount of distribution in the Korean Peninsula, Siberia and Japan. Its dried corm is used as medicine, sweet, pungent, cold, slightly poisonous. It has the effect of nourishing yin and clearing lung, resolving phlegm and relieving cough. It is used for carbuncle, scrofula, and unknown swelling. However, the medicinal value of Shanlan has not been valued for a long time. Or used as medicine. In recent years, with the increase in demand and the skyrocketing price of Shanlan, its wild resources have been destroyed, and it has been listed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张正海张亚玉孙海张悦
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS