Inversion method for microcystin MC-LR concentration in water body

A technology of microcystin and MC-LR, which is applied in the direction of measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems that the inversion model cannot be used universally, and achieve the effect of rapid detection, high accuracy and good stability

Active Publication Date: 2019-08-30
YANGZHOU UNIV
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the inversion m

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Inversion method for microcystin MC-LR concentration in water body
  • Inversion method for microcystin MC-LR concentration in water body
  • Inversion method for microcystin MC-LR concentration in water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] Below in conjunction with specific embodiment, further illustrate the present invention.

[0033] The detection method of microcystin MC-LR in water body, including:

[0034] (1) Sampling: during the outbreak period of Microcystis aeruginosa, 1L water samples were taken with a sampler at a monitoring point of Wuxi Taihu Lake at a depth of 20-30cm, and the number of samples was 31. Nine samples were randomly selected as samples to be inverted, and other samples were used to construct the inversion equation.

[0035] (2) With reference to the national standard GB / T20466-2006 method, the Agilent 1200 liquid chromatograph was used to determine the content of microcystin MC-LR in the water sample, which was used for the establishment of the inversion equation and inversion detection;

[0036] (3) Extract the extracellular organic matter of Microcystis aeruginosa in the water sample: the extracellular organic matter of Microcystis aeruginosa is divided into two parts: dEOM (...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an inversion method for microcystin MC-LR concentration in a water body. The method comprises the steps of establishing a unary linear inversion equation by taking normalized characteristic fluorescence peak intensity of a microcystis aeruginosa extracellular organic matter in a water sample as an independent variable and normalized microcystin MC-LR concentration in the water sample as a dependent variable, wherein the characteristic fluorescence peak intensity and the microcystin MC-LR concentration are normalized through utilization of chlorophyll a concentration inthe water sample; obtaining a to-be-detected water sample, measuring three-dimensional excitation-emission matrix fluorescence spectrum of the microcystis aeruginosa extracellular organic matter in the water sample, and obtaining the characteristic fluorescence peak intensity of the microcystis aeruginosa extracellular organic matter; and substituting the characteristic fluorescence peak intensityin to the unary linear inversion equation, and obtaining the microcystin MC-LR concentration. According to the method, sample pretreatment is avoided, rapid online monitoring of the microcystin MC-LRconcentration can be realized, the characteristic fluorescence peak intensity of the microcystis aeruginosa extracellular organic matter is taken as the variable for establishment of the equation, and a new thought for the inversion method is provided.

Description

technical field [0001] The invention relates to water body environment detection, in particular to a method for retrieving the concentration of microcystin MC-LR in water body. Background technique [0002] A large amount of microcystin MC-LR enters the water body, which seriously threatens the safety of drinking water. Microcystin MC-LR is mainly produced by Microcystis aeruginosa in water. At present, conventional concentration detection methods are divided into biological detection methods, physical chemical detection methods, biochemical detection methods, and molecular biological detection methods. The pretreatment of water samples for each detection method is cumbersome, time-consuming, and costly, and rapid online monitoring cannot be achieved. The inversion method can use the influencing factors highly related to the concentration of MC-LR to construct an inversion model, which can indirectly reflect the change of the concentration of microcystin MC-LR in the water ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 王志刚王赛赛张晴静孙宇
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap