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Cardia adenocarcinoma auxiliary diagnosis kit related to a group of genes

A technique for assisting the diagnosis of cardiac adenocarcinoma, which is applied in the fields of biomedicine and molecular biology, and can solve difficult clinical problems, low specificity, and low sensitivity

Active Publication Date: 2019-08-30
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional tumor markers often have disadvantages such as specificity and low sensitivity, and are difficult to be widely used in clinical practice.

Method used

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  • Cardia adenocarcinoma auxiliary diagnosis kit related to a group of genes
  • Cardia adenocarcinoma auxiliary diagnosis kit related to a group of genes
  • Cardia adenocarcinoma auxiliary diagnosis kit related to a group of genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of embodiment 1 specimen

[0038] 1.1 Specimen source

[0039] From October 2014 to December 2017 in Anyang Cancer Hospital, 651 patients diagnosed with cardia adenocarcinoma were selected and included in the patient group. The inclusion criteria are as follows:

[0040] (1) Cases undergoing surgical resection;

[0041] (2) Cardia adenocarcinoma diagnosed by postoperative pathology with corresponding paracancerous tissue, which is defined as cardiac tissue beyond 5cm from the tumor edge;

[0042] (3) None of the patients received preoperative neoadjuvant therapy.

[0043] Select the same number of healthy volunteers as a group of healthy volunteers. "Healthy volunteers" were defined as volunteers who did not find any pathological changes in the upper gastrointestinal tract, including positive changes, after gastroscopy.

[0044] 1.2 Patient group tissue samples

[0045] In the patient group, after surgical resection of cardiac adenocarcinoma tissue...

Embodiment 2

[0050] Example 2 RT-PCR method to study the expression difference of genes BCL2L1, DLC1, CDKN2A, CDKN2B, NRG1, Erb B4 in cardia adenocarcinoma and adjacent tissues

[0051] 2.1 Experimental method

[0052] 2.1.1 RNA extraction from tissue samples

[0053] The cardia adenocarcinoma tissues and corresponding paracancerous tissues derived from the patient group in Example 1 were respectively subjected to RNA extraction according to the numbers, and the cardiac adenocarcinoma tissues and corresponding paracancerous tissues of each case were grouped into one group, and the cardiac adenocarcinoma tissues were used as The experimental samples and the corresponding paracancerous tissues were used as control samples, and 651 patients were divided into 651 groups.

[0054] Reagent (invitrogen, Cat. No. 15596-018) was used to extract RNA from 651 groups of samples, and the experimental operation was carried out according to the product instructions. The specific operation of each group ...

Embodiment 3

[0076] Example 3 Study of the expression differences of genes BCL2L1, DLC1, CDKN2A, CDKN2B, NRG1, and Erb B4 in cardia adenocarcinoma and adjacent tissues by immunohistochemical staining

[0077] 3.1 Tissue staining

[0078] The cardiac adenocarcinoma tissue and paracancerous tissue samples of 651 patients in the patient group were processed as follows:

[0079] The specimens were fixed with 4% paraformaldehyde at room temperature for 24h to 72h, embedded in paraffin, and made into 5mm sections. Paraffin sections were dewaxed in xylene, dehydrated in graded ethanol, and placed in 0.3% H 2 o 2 -Incubate in methanol solution for 30 minutes at room temperature to eliminate endogenous peroxidase activity, wash with distilled water, and retrieve antigen by microwave method, soak in phosphate buffered saline (PBS) for 5 minutes, place in 1:10 goat serum blocking solution and incubate at room temperature for 10 minutes.

[0080] Each sample obtained after the above steps was rando...

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Abstract

The invention relates to a cardia adenocarcinoma auxiliary diagnosis kit related to a group of genes, belongs to the technical field of biomedicine and molecular biology, and particularly relates to an ELISA kit for auxiliary diagnosis of cardia adenocarcinoma. The kit comprises a solid phase carrier and six antibodies which are respectively coated on the solid phase carrier, wherein the six antibodies are as follows: BCL2L1 antibody, DLC1 antibody, CDKN2A antibody, CDKN2B antibody, NRG1 antibody, ErbB4 antibody. Experiments prove that the group of related gene expression product has high expression in the serum of a cardia adenocarcinoma patient, the expression level of the related gene in the human serum is detected by the ELISA kit, and the detection result can be used for auxiliary diagnosis of the cardia adenocarcinoma such as early-onset screening, prognosis evaluation and the like, and the ELISA kit for auxiliary diagnosis of cardia adenocarcinoma has profound clinical significance and important popularization and application prospect.

Description

technical field [0001] The invention relates to a kit for early screening or auxiliary diagnosis of cardia adenocarcinoma by detecting a group of gene expression levels, belonging to the technical fields of biomedicine and molecular biology. Background technique [0002] Gastric Cardia Adenocarcinoma (GCA) is a malignant tumor located in the anatomical position of the human cardia, which endangers people's quality of life and life, and brings a heavy burden to the patient's family and the country. In the past few decades, the incidence of GCA in high-incidence areas in my country has been increasing year by year, increasing by seven times, and the incidence of GCA is higher than other malignant tumors. Henan Province in my country has the highest incidence rate of cardia adenocarcinoma in the world, reaching 190 / 100,000, and the incidence rate is still rising. [0003] Previous studies have identified several risk factors associated with the development of GCA, including li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57446
Inventor 王立东李欣然宋昕赵学科胡守佳郭海周郑颖娟
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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