Fluorescence service life characterization method for pulse delay estimation

Abstract

Provided in the invention is a fluorescence service life characterization method for pulse delay estimation. The method includes the following steps: step one, measuring a time-domain continuous forward dispersion light pulse signal and a time-domain continuous fluorescence pulse signal that are obtained by cell irradiation by laser in a flow cytometry; step two, calculating refined frequency spectrums for time-domain continuous pulse signals by using improved chirp Z-transform, carrying out equal-angle sampling along a spiral curve, calculating limited time width Z transform, and converting the time domain signals into frequency domains to obtain discrete frequency-domain signals; step three, carrying out multiplying on the obtained frequency domains and carrying out zero filling, improving the resolutions of cross-correlation peaks by using a correlation peak interpolation algorithm, and minimizing a peak point interval to obtain a time domain cross-correlation function; and step four, calculating two signal delays based on the time domain cross-correlation function to characterize the fluorescence service life.

Description

technical field [0001] The method of the invention discloses a fluorescence lifetime characterization method of stained cells in a flow cytometer, and belongs to the field of biomedical detection. Background technique [0002] Flow cytometry detects multicolor fluorescent signals by irradiating lasers with specific wavelengths to stimulate fluorescent intensity signals, including forward scattered light, side scattered light and various fluorescent signals, and perform high-speed quantitative analysis on them , have been more and more widely used in biochemistry, medicine, industrial detection and chemical research. As an intrinsic parameter of the fluorescence signal, the fluorescence lifetime is not easily disturbed by external factors, and has better stability and measurement accuracy. Its accurate measurement is of great significance for fluorescence detection and analysis technology. [0003] There are three main types of traditional fluorescence lifetime measurement m...

AI Technical Summary

Problems solved by technology

However, most of these methods require complex and expensive instruments, which greatly increase the cost and complexity of measuring fluorescence lifetime.

In recent years, Cao et al. proposed that without changing the structure of the flow cytometry analysis system, a high-speed ADC chip is used to sample the fluorescence signal and an algorithm is used to calculate the time delay of the fluorescence. However, the sampling frequency of the ADC cannot be increased infinitely, so the time-domain resolution of the calculation results of this method is limited

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