Molecular marker for diagnosing atherosclerosis and application of marker

Abstract

The invention discloses a molecular marker for diagnosing atherosclerosis and an application of the marker, belongs to the technical field of biological engineering and relates to an application of areagent for detecting the expression level of SCRAB1 in blood vessel samples to preparation of products for diagnosing the atherosclerosis, and A sequence of the SCRAB1 is as shown in SEQ: ID: NO: 1.ApoE knockout mice and THP-1 source foam cells serve as research objects, RNA (ribonucleic acid) of tissues or cells is extracted, expression change of the gene SCRAB1 related to AS lipid is observed,cellular level validation is implemented, and a deposition specificity diagnosis method of the AS lipid comprising the SCRAB1 is built. The method is simple and reliable, materials are conveniently taken, sensitivity and specificity of the method are highly consistent with those of imaging diagnosis and gross specimen staining, and the method has high application values in early diagnosis and prognosis evaluation of AS diseases.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a molecular marker for diagnosing atherosclerosis and its application, in particular to a method for diagnosing homocysteine ​​(Hcy)-associated atherosclerosis (AS). ) Lipid metabolism markers and their applications. Background technique [0002] Atherosclerosis (AS) is a systemic vascular disease related to lipid metabolism disorder, which is one of the most important pathophysiological basis of vascular obstructive diseases such as acute myocardial infarction and cerebral infarction in clinical practice. Important topic in the field of medical research. With the acceleration of the population aging process, the number of patients with hypertension, hyperlipidemia, and hyperhomocysteine ​​(Hcy) is increasing year by year, and the onset of AS is becoming younger, which brings a heavy burden to families and society. Furthermore, atherosclerotic plaque formation is a comp...

AI Technical Summary

Problems solved by technology

However, there is still little research on biomarkers related to lipid metabolism in atherosclerotic plaques, especially those that are differentially expressed in atherosclerotic plaques.

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