A method for constructing agarwood digital chromatography/mass spectrometry fingerprint

Abstract

The invention discloses a method for constructing an agilawood digitized chromatography / mass spectrometry fingerprint. The method uses high performance liquid chromatography / time of flight mass spectrometry to determine the agilawood extract, obtains a liquid chromatogram and a total ion chromatogram of the high resolution mass spectrum, and characterizes each chemical component of the agilawood according to the retention time of chromatographic peaks and the precise mass number of the high resolution mass spectrum corresponding to each chromatographic peak, that is, each component gives a unique digital information and the chemical compositions are characterized in a digital form, thereby establishing a digitized HPLC-UV / TOF MS chromatogram / high resolution mass spectrometry fingerprint ofagilawood. The method provided by the invention gives digital identification information similar to identity identification for each chemical composition of agilawood, has uniqueness, and can comprehensively reflect the material composition of agilawood and realize comprehensive and scientific characterization of agilawood.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to a method for constructing digital chromatographic / mass spectrum fingerprints of agarwood. Background technique [0002] At present, there are the following problems in Chinese medicinal materials: one is that the identification of Chinese medicinal materials stipulated in the "Chinese Pharmacopoeia" mostly adopts thin-layer chromatography and liquid chromatography, which can only be judged by retention time, and the results are not accurate enough; The basis is the chemical composition, but there are still many components that have not been clarified, and the qualitative data of the chemical components corresponding to each chromatographic peak cannot be given, which brings difficulties to the quality evaluation and quality control of traditional Chinese medicine, and affects the modernization process of traditional Chinese medicine. [0003] Agarwood is produced...

AI Technical Summary

Problems solved by technology

[0002] At present, there are the following problems in Chinese medicinal materials: one is that the identification of Chinese medicinal materials stipulated in the "Chinese Pharmacopoeia" mostly adopts thin-layer chromatography and liquid chromatography, which can only be judged by retention time, and the results are not accurate enough; The basis is the chemical composition, but there are still many components that have not been clarified, and the qualitative data of the chemical components corresponding to each chromatographic peak cannot be given, which brings difficulties to the quality evaluation and quality control of traditional Chinese medicine, and affects the modernization process of traditional Chinese medicine.

There are few relevant reports on the analysis of chemical components of agarwood at home and abroad. The quantitative index of agarwood in the "Chinese Pharmacopoeia" is only agarwood tetraol. However, there are many components of agarwood that work together. A method for comprehensive, scientific characterization of chemical constituents

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