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Biological enzyme processing method for seaweed beverage processing

A processing method and bio-enzyme technology, which is applied in the field of bio-enzyme processing for seaweed beverage processing, can solve the problems of reducing the loss of nutrients, high cost, and low yield of extraction technology, so as to reduce loss, low cost, and solve the problem of excessive yield. low effect

Inactive Publication Date: 2019-09-13
SHISHI HUABAO MINGXIANG FOODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] When using seaweed to make seaweed beverage, it is necessary to extract bioactive components such as trehalose in seaweed to reduce the loss of nutrients. The existing extraction technology has a low yield and high cost. Therefore, we propose a seaweed beverage. biological enzyme processing method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A biological enzyme processing method for seaweed beverage processing, comprising the steps of:

[0024] S1. Take out the seaweed from the freezer, thaw it in the refrigerator at 4°C, and beat the seaweed with a crusher;

[0025] S2. Weigh 100g of seaweed slurry, add 6ml of compound enzyme solution into it, heat and react in a water bath for 2 hours, use it and then adjust it to 90°C for 30 minutes to activate the enzyme;

[0026] S3. Centrifuge at 3000r / min for 10min, take out the supernatant, add 95% ethanol solution, let stand, and fully precipitate;

[0027] S4. Take out the supernatant, centrifuge the remaining liquid for 15 minutes again, wash the precipitate with absolute ethanol, obtain trehalose after drying, and weigh its mass.

[0028] This technical scheme uses compound enzymatic hydrolysis technology to extract biologically active components in seaweed, reducing the loss of nutrients, and the compound enzyme is glucose phosphorylase and trehalose phosphory...

Embodiment 2

[0030] A biological enzyme processing method for seaweed beverage processing, comprising the steps of:

[0031] S1. Take out the seaweed from the freezer, thaw it in the refrigerator at 4°C, and beat the seaweed with a crusher;

[0032] S2. Weigh 100g of seaweed pulp, add 6ml of compound enzyme solution into it, heat and react in a water bath for 2 hours, use it and then adjust it to 90°C for 30 minutes to promote enzyme activation, and seal the reaction container with plastic wrap during the enzymatic hydrolysis process , to prevent water evaporation; after enzymatic hydrolysis, quickly cool down to room temperature with cold water;

[0033] S3. Centrifuge at 3000r / min for 10min, take out the supernatant, add 95% ethanol solution, let stand, and fully precipitate;

[0034] S4. Take out the supernatant, centrifuge the remaining liquid for 15 minutes again, wash the precipitate with absolute ethanol, obtain trehalose after drying, and weigh its mass.

[0035] This technical s...

Embodiment 3

[0037] A biological enzyme processing method for seaweed beverage processing, comprising the steps of:

[0038] S1. Take out the seaweed from the freezer, thaw it in the refrigerator at 4°C, and beat the seaweed with a crusher;

[0039] S2. Weigh 100g of seaweed pulp, add 6ml of compound enzyme solution into it, heat and react in a water bath for 2 hours, use it and then adjust it to 90°C for 30 minutes to promote enzyme activation, and seal the reaction container with plastic wrap during the enzymatic hydrolysis process , to prevent water from evaporating; after enzymolysis, cool it down to room temperature quickly with cold water, and use the seaweed juice that was directly squeezed and filtered without adding enzyme after seaweed beating as the control group;

[0040] S3. Centrifuge at 3000r / min for 10min, take out the supernatant, add 95% ethanol solution, let stand, and fully precipitate;

[0041] S4. Take out the supernatant, centrifuge the remaining liquid for 15 minut...

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PUM

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Abstract

The present invention discloses a biological enzyme processing method for seaweed beverage processing. The biological enzyme processing method comprises the following steps: S1, seaweed is taken out from a freezer and thawed in a refrigerator in an environment of 4 DEG C, and a crusher is used to beat the seaweed into slurry; S2, 100 g of the seaweed slurry is weighed, 6 ml of a compound enzyme solution is added, the mixture is subjected to a heating reaction in a water bath pot for 2 h, then a temperature is adjusted to 90 DEG C, and the heating reaction continues for 30 min to promote enzymeactivation; S3, centrifugation is conducted at a condition of 3,000 r / min for 10 min, a supernatant is taken out, and 95% of an ethanol solution is added; and S4, the supernatant is taken out, remaining liquid is then centrifuged again for 15 min, and a precipitate is washed with absolute ethanol. The compound enzymatic hydrolysis technology is used to extract biological active components in theseaweed and reduce loss of nutrients, besides, the compound enzyme is glucose phosphorylase and trehalose phosphorylase using glucose as a substrate, yield of extracted trehalose is up to 7.2, an extraction process is simple, low in costs and high in efficiency, and the biological enzyme processing method solves problems that existing extraction technology is too low in yield and also relatively high in costs.

Description

technical field [0001] The invention relates to the technical field of seaweed beverage processing, in particular to a biological enzyme processing method for seaweed beverage processing. Background technique [0002] "Seaweed" is the general term for marine algae such as kelp, seaweed, wakame, and aquamarine. It is an algae that grows in the sea and is a cryptogamous plant in the plant kingdom. Algae include several different types of organisms that generate energy through photosynthesis. They are generally considered to be simple plants, and the main features are: no vascular tissue, no real differentiation of roots, stems, and leaves; no flowering, no fruits and seeds, no specialized protective tissues for reproductive organs, and often directly formed by a single plant. The cells produce spores or gametes, and no embryos form. [0003] Trehalose is also known as leaking sugar, mushroom sugar and so on. It is a safe and reliable natural sugar. Trehalose is a non-reduci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12
CPCC12P19/12
Inventor 刘鹏飞
Owner SHISHI HUABAO MINGXIANG FOODS