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Method for rapidly detecting wild cyprinus carpio spawning grounds

A technology of Yellow River carp and spawning grounds, applied in biochemical equipment and methods, microbe measurement/inspection, etc., can solve problems such as costing a lot of manpower, and achieve the effect of saving manpower, material resources and financial resources

Pending Publication Date: 2019-09-13
XIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the Yellow River is very huge, the spawning grounds of the Yellow River carp are not fixed every year, so researchers need to spend a lot of manpower and material resources to accurately find the spawning grounds of the Yellow River carp

Method used

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  • Method for rapidly detecting wild cyprinus carpio spawning grounds
  • Method for rapidly detecting wild cyprinus carpio spawning grounds
  • Method for rapidly detecting wild cyprinus carpio spawning grounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1: According to the breeding habits of Yellow River carp, during the carp breeding season in March, screen the location of suspected carp spawning grounds, set 2 sampling points every 6 kilometers, and set 4 sampling points in the cross-section of the Yellow River at each sampling point. When sampling at each sampling location, the surface, middle and lower layers of the Yellow River were collected three times. Sampling must be done at the same time. Each sampling point must be sampled 3 times consecutively.

[0028] Step 2: Suction filter the collected Yellow River water sample (according to the machine, the air pressure in the suction filter bottle is lowered, and the water sample enters the water suction filter bottle through the suction filter paper, but the DNA in the water sample remains on the paper) , and the filter membrane after suction filtration was subjected to DNA extraction.

[0029] Step 3: Perform fluorescence quantitative PCR analysis on the DNA...

Embodiment 2

[0035] Step 1: According to the breeding habits of Yellow River carp, during the carp breeding season in April, screen the location of suspected carp spawning grounds, set 3 sampling points every 6 kilometers, and set 4 sampling points in the cross-section of the Yellow River at each sampling point. When sampling at each sampling location, the surface, middle and lower layers of the Yellow River were collected three times. Sampling must be done at the same time. Each sampling point shall be sampled 4 times consecutively.

[0036] Step 2: Suction filter the collected Yellow River water sample (according to the machine, the air pressure in the suction filter bottle is lowered, and the water sample enters the water suction filter bottle through the suction filter paper, but the DNA in the water sample remains on the paper) , and the filter membrane after suction filtration was subjected to DNA extraction.

[0037] Step 3: Perform fluorescence quantitative PCR analysis on the DN...

Embodiment 3

[0043] Step 1: According to the breeding habits of Yellow River carp, during the carp breeding season in May, screen the location of suspected carp spawning grounds, set 4 sampling points every 6 kilometers, and set 5 sampling points in the cross-section of the Yellow River at each sampling point. When sampling at each sampling location, the surface, middle and lower layers of the Yellow River were collected three times. Sampling must be done at the same time. Each sampling point must be sampled 3 times consecutively.

[0044] Step 2: Suction filter the collected Yellow River water sample (according to the machine, the air pressure in the suction filter bottle is lowered, and the water sample enters the water suction filter bottle through the suction filter paper, but the DNA in the water sample remains on the paper) , and the filter membrane after suction filtration was subjected to DNA extraction.

[0045] Step 3: Perform fluorescence quantitative PCR analysis on the DNA e...

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Abstract

The invention discloses a method for rapidly detecting wild cyprinus carpio spawning grounds. The method particularly includes the followings steps: 1, a Yellow River region is searched for sites likely to be the spawning grounds, the sites serve as detection objects, and in a cyprinus carpio breeding season, water sampling is conducted at locations of the detection objects; 2, collected water samples are subjected to suction filtration, and DNA on filter membranes is extracted after suction filtration; and 3, extracted DNA is subjected to fluorescent quantitative PCR analysis, according to analysis results, the expression contents of target genes of all the sampling locations are compared, and the sampling locations with high expression contents of the target genes are locations of the cyprinus carpio spawning grounds. According to the method for rapidly detecting the wild cyprinus carpio spawning grounds, by detecting the contents of the target genes in the water samples, the locations of the cyprinus carpio spawning grounds can be rapidly and accurately identified. Compared with traditional methods for looking for the spawning grounds, the method has the advantages that manpower, material resources and financial resources are greatly saved.

Description

technical field [0001] The invention belongs to the technical field of animal molecular breeding methods, in particular to a method for quickly detecting the spawning grounds of wild Yellow River carp. Background technique [0002] The Yellow River is the second largest river in my country, with a total length of 5,464 kilometers and a drainage area of ​​750,000 square kilometers. It is known as "copper head, iron tail, and tofu waist". It runs through Henan for 692 kilometers and is mainly located on the tofu waist. The river channel to the east of Mengjin begins to widen. The distance between the dikes on both banks is generally about 10 kilometers, and the widest part can reach 20 kilometers. salt. The watershed (Henan section) has a mild climate, long annual sunshine hours and long fish growth seasons, and a large number of wild weeds growing on the Yellow River beach can be used as fish feed, all of which are favorable conditions for the development of fishery producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851C12Q1/6888
CPCC12Q1/6851C12Q1/6888C12Q2531/113C12Q2563/107
Inventor 王俊潘保柱蒋小明孙智薇
Owner XIAN UNIV OF TECH
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