Reagent kit for detecting streptococcus pyogenes

Abstract

The invention provides a reagent kit for detecting streptococcus pyogenes. The reagent kit contains a guide RNA specifically targeted to a streptococcus pyogenes rpoB gene, an amplification primer pair, a hydration TwistAmp basic kit reaction drying ball, LbCas12a protein, a single-stranded DNA probe (ssDNA), a Ribonuclease Inhibitor and a buffer solution. When the reagent kit is used for detecting the streptococcus pyogenes, the detection specificity is high, and the streptococcus pyogenes can be distinguished from other streptococcus including streptococcus dysgalactiae and streptococcus agalactiae. Besides, when the RNA sequence is used for detection, time consumption is about 1 hour, a PCR instrument is not needed, requirements for equipment are low and are notably lower than those by a traditional bacteria culture method or a PCR sequencing method, and the clinical use value is large.

Description

technical field [0001] The invention belongs to the field of detection of pathogenic bacteria, and relates to a kit for detection of Streptococcus pyogenes. This kit can be used for rapid detection of Streptococcus pyogenes. Background technique [0002] Streptococcus pyogenes is a common bacterial group among Gram-positive bacteria that infects human beings, which brings a huge burden to patients and society. The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR is often unable to distinguish Streptococcus pyogenes from other Streptococcus due to the lack of specific primers, so joint generation sequencing is required. Identification is expensive and takes several days to produce results. During this period, clinicians can only use medicines based on experience because they do not obtain test results, which often leads to delays in disease treatment. Therefore, how to quickly detect Streptococcus p...

AI Technical Summary

Problems solved by technology

The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Streptococcus pyogenes from other Streptococci due to the lack of specific primers, so joint generation sequencing is required to Identification, high cost, takes several days to get results

During this period, clinicians can only use medicines based on experience because they have not obtained test results, which often leads to delays in disease treatment

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