HPLC method for measuring seven organic acids in jackinthepulpit tuber simultaneously

A technology of organic acids and detection methods, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult quality, and achieve the effects of high accuracy, good specificity and simple operation

Active Publication Date: 2019-09-17
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is well known that the pleiotropic effects of traditional Chinese medicine stem from its complex chemical components, and the determination

Method used

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  • HPLC method for measuring seven organic acids in jackinthepulpit tuber simultaneously
  • HPLC method for measuring seven organic acids in jackinthepulpit tuber simultaneously
  • HPLC method for measuring seven organic acids in jackinthepulpit tuber simultaneously

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The quantitative assay method of embodiment 1 Rhododendron chinensis medicinal material

[0033] 1. Preparation of reference substance solution: Accurately weigh an appropriate amount of reference substances of oxalic acid, L-malic acid, citric acid, succinic acid, fumaric acid, cis-aconitic acid, and trans-aconitic acid, and add water to make each 1mL containing A mixed reference solution of 200 μg oxalic acid, 100 μg L-malic acid, 200 μg citric acid, 200 μg succinic acid, 5 μg fumaric acid, 10 μg cis-aconitic acid, and 10 μg trans-aconitic acid.

[0034] 2. Preparation of the test solution: Take about 0.5g of the sample powder (passed through a No. 4 sieve), accurately weigh it, add 50mL of water, weigh it, treat it ultrasonically for 60 minutes, let it cool, weigh it again, and make up the lost weight with water , filter or centrifuge (10000r / min, centrifuge for 5min, take the supernatant and centrifuge again), accurately measure 5mL of the supernatant, add 1mL of ph...

Embodiment 2

[0038] Example 2 Methodological investigation of the determination method of the medicinal material content of Rhododendron chinensis

[0039] 1. Linear relationship investigation: Accurately absorb the mixed reference substance solution in Example 1, add water to dilute into a series of mixed reference substance solutions of different concentrations, inject 20 μ L of sample, measure, record the chromatogram, measure its peak area, and use the reference substance The concentration (μg / mL) is the abscissa (X), and the peak area is the ordinate (Y) to draw a standard curve to obtain a regression equation. The results are shown in Table 1.

[0040] Table 1 Linear relationship inspection results

[0041]

[0042] The results showed that the reference substances of oxalic acid, L-malic acid, citric acid, succinic acid, fumaric acid, cis-aconitic acid and trans-aconitic acid had a good linear range.

[0043] 2. Precision test: Precisely draw the mixed reference substance solutio...

Embodiment 3

[0047] The determination of the content of the sample of embodiment 3 Rhododendron chinensis

[0048] Get 0.5g of Tiger Palm Southern Star sample powder (crossing No. 4 sieve), accurately weighed, prepare need testing solution by the method in Example 1, carry out quantitative analysis to 7 kinds of organic acids by the chromatographic condition in Example 1, determine The peak area of ​​each component was substituted into the regression equation to calculate the content of each organic acid, and the results are shown in Table 2.

[0049] Table 2 Determination results of 7 kinds of organic acids in 11 batches of Nanxing Huzhang (n=2)

[0050]

[0051] To sum up, the HPLC detection method of the medicinal material Nanxing Polygonatum of the present invention can not only detect 4 kinds of organic acid components in the medicinal material Nanxing Polygonatum simultaneously: oxalic acid, L-malic acid, citric acid and fumaric acid, but also can simultaneously detect other Thre...

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Abstract

The invention provides an HPLC method for measuring four organic acids (oxalic acid, L-malic acid, citric acid and fumaric acid) in jackinthepulpit tuber simultaneously. Another three organic acids (succinic acid, cis-aconitic acid and trans-aconitic acid) also can be measured simultaneously in the method. According to the HPLC measuring method provided by the invention, various organic acid ingredients in the medicinal material of jackinthepulpit tuber can be measured simultaneously, the method is simple in operation, high in sensitivity, high in specificity and high in accuracy, and the quality of the medicinal material of jackinthepulpit tuber can be evaluated effectively.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine analysis, and in particular relates to an HPLC method for simultaneously determining seven organic acids in the tiger's palm nanxing. Background technique [0002] Tiger palm Nanxing is derived from the dry tuber of Pinellia pedatisecta Schott, a plant of the genus Pinellia in the Araceae family Pinellia genus. Treatment of stroke with phlegm congestion, crooked mouth and eyes, hemiplegia, epilepsy, convulsions, tetanus, wind-phlegm dizziness, sore throat, scrofula, carbuncle, tumbling injuries, snake bites. [0003] There have been many reports on the determination of the content of Pinellia chinensis and its congener plants, but only 2 to 4 components were determined. It is well known that the pleiotropic effect of traditional Chinese medicine stems from its complex chemical components, and the determination of the content of only one or two components cannot reflect the si...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李敏敬勇赖月月陈华林刘佳灵李巧
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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