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Application of brassica napus short peptide BnPEP5

A short peptide, rapeseed technology, applied in the biological field, can solve the problems of human and animal poisoning, ecological pollution, drug resistance of pathogens, etc., and achieves the effects of low concentration, strong disease resistance stimulation, and easy acquisition.

Active Publication Date: 2019-10-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because some pesticides have problems such as ecological pollution, human and animal poisoning, and easy resistance of pathogens, new green control agents are urgently needed to be developed

Method used

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  • Application of brassica napus short peptide BnPEP5
  • Application of brassica napus short peptide BnPEP5
  • Application of brassica napus short peptide BnPEP5

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 BnPEP5 short peptide in rapeseed ( Brassica napus ) application in antibacterial sclerotinia

[0019] In the embodiment of the present invention, artificially synthesized BnPEP5 short peptide is used to treat rapeseed by soaking or spraying to stimulate its resistance to Sclerotinia sclerotiorum, so as to control Sclerotinia sclerotiorum. The main steps include:

[0020] 1) Artificial synthesis of BnPEP5 short peptide

[0021] The BnPEP5 short peptide sequence provided by the present invention is shown in SEQ ID: 1, consisting of 27 amino acids in total, and is the C-terminal part of the full-length protein (sequence shown in SEQ ID: 2). Prior to the present invention, there were not any published reports on the function of the short peptide BnPEP5.

[0022] In this example, a professional peptide synthesis company (Qiangyao Biotechnology Co., Ltd.) was entrusted to synthesize BnPEP5 short peptides for the analysis and application of rapeseed disease resis...

Embodiment 2

[0029] Example 2 BnPEP5 short peptide in mustard ( Brassica juncea ) application in antibacterial sclerotinia

[0030] In the embodiment of the present invention, artificially synthesized BnPEP5 short peptide is used to treat mustard by soaking or spraying to stimulate its resistance to Sclerotinia sclerotiorum, so as to control Sclerotinia mustard. The main steps include:

[0031] 1) Artificial synthesis of BnPEP5 short peptide

[0032] The BnPEP5 short peptide sequence provided by the present invention is shown in SEQ ID: 1, consisting of 27 amino acids in total, and is the C-terminal part of the full-length protein (sequence shown in SEQ ID: 2). In this example, a professional peptide synthesis company (Qiangyao Biotechnology Co., Ltd.) was entrusted to synthesize BnPEP5 short peptides for the analysis and application of mustard disease resistance stimulation and Sclerotinia prevention and control functions.

[0033] 2) BnPEP5 short peptide treatment of mustard

[0034...

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Abstract

The invention provides application of brassica napus short peptide BnPEP5 in stimulating cruciferous plants to have resistance to sclerotinia sclerotiorum. The brassica napus short peptide BnPEP5 comprises 27 amino acids and is a C-terminal portion of full-length protein. The short peptide BnPEP5 is synthesized artificially for the first time, the stimulation effect of the short peptide on the resistance to the sclerotinia sclerotiorum is illustrated, and the short peptide is applied to stimulate the cruciferous plants, including the brassica napus and brassica juncea, to have the resistance to the sclerotinia sclerotiorum through infiltrating and spraying to control sclerotinia of the brassica napus and the brassica juncea. The short peptide BnPEP5 is a product self-synthesized from plants, is used for stimulating immunity to prevent the sclerotinia, and has the advantages of naturality, no environment pollution, harmlessness to human and livestock, incapability of inducing pathogensto have drug resistance, and the like. The short peptide BnPEP5 is easy to synthesize and simple and convenient to obtain and has a strong stimulation effect on disease resistance, and the required concentration of the BnPEP5 is low, so that the BnPEP5 is a novel ecological and environment-friendly crop disease control agent.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to the immune stimulating function of a short rapeseed peptide BnPEP5 and its application in the prevention and control of sclerotinia. Background technique [0002] 1. Plant immune stimulation technology [0003] Plant immunity is a defense response activated by plants through receptor recognition of pathogenic molecules. The plant immune system has multiple levels, among which the first level is the immunity produced by the transmembrane molecular pattern recognition receptors (pattern recognition receptors, PRRs) on the surface of plant cells to recognize the conserved molecular patterns derived from pathogens and plants themselves, called pattern Triggered immunity (pattern-triggered immunity, PTI). In layman's terms, plants can perceive the destruction of plant cell integrity caused by pathogen infection, etc., and produce damage-associated molecular patterns (DAMPs), which...

Claims

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Application Information

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IPC IPC(8): A01N47/44A01N37/46A01P3/00C07K14/415
CPCA01N37/46A01N47/44C07K14/415
Inventor 蔡新忠徐幼平叶云峰
Owner ZHEJIANG UNIV
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