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Method for preventing and controlling chilo suppressalis by entomopathogenetic nematodes

A technology for entomopathogenic nematodes and pathogenic nematodes, applied in the directions of botanical equipment and methods, applications, pesticides, etc., can solve the problems of unsatisfactory effect of Bacillus thuringiensis, increased resistance of pests, non-point source pollution of pesticides, etc. The effect of strong search function, simple application method and high infection efficiency

Inactive Publication Date: 2019-10-08
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The large-scale use of chemical pesticides has not only caused non-point source pollution of pesticides, but also caused pesticide residues in agricultural products to exceed the standard, and the resistance of pests has increased significantly
Due to the harm of rice borer in the larval stage, the widely used biopesticide Bacillus thuringiensis is not ideal, and it is urgent to develop other high-efficiency biopharmaceuticals

Method used

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  • Method for preventing and controlling chilo suppressalis by entomopathogenetic nematodes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of the pathogenic nematode mixture: soak the pathogenic nematode preparation in clear water, and gently stir to release the pathogenic nematodes and suspend them in the water. Add about 100L of water for every 20 million entomopathogenic nematodes to dilute, and make a concentration of 200IJs / ml of entomopathogenic nematode mixture.

Embodiment 2

[0023] Indoor activity test of pathogenic nematodes: put filter paper in each petri dish, select 10 3rd, 4th and 5th instar larvae of Chilo borer respectively and place them in separate petri dishes, and set 3 groups of controls and replicates for each treatment . Add 1.5ml of the diluted pathogenic nematode mixture into each petri dish, place it at 26°C and culture it for 2-3 days, and check the survival rate of Chilo suppressalis larvae. The results show that the control effect of the pathogenic nematodes on the 3rd instar larvae of Chilo borer reaches 70%, and the control effect on the 5th instar larvae reaches more than 50%. The indoor control effects of entomopathogenic nematodes on Chilo borer are shown in Table 1.

[0024] Table 1 Indoor control effects of entomopathogenic nematodes on Chilo borer

[0025]

Embodiment 3

[0027] The choice of field application time: According to the forecast of the local agricultural technology extension department, select the period of high incidence of young larvae, apply the pathogenic nematode preparation in the evening or on cloudy days, keep 5cm of water in the field, and avoid application when the sun is strong, windy and rainy, or the field is dry. .

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Abstract

The invention belongs to the field of biological control of agricultural insect, and particularly discloses a method for preventing and controlling chilo suppressalis by entomopathogenetic nematodes.The method includes preparing mixed liquor of entomopathogenetic nematodes, applying the mixed liquor of entomopathogenetic nematodes in the larval phase of chilo suppressalis, holding water on filedfor two days, infecting chilo suppressalis in rice stems due to the fact that entomopathogenetic nematodes can search hosts autonomously, thereby controlling hazards of chilo suppressalis. The methodrefers to the nuisanceless chilo suppressalis biological control technology for rice, has the advantages of simple application, makes full use of high searching function and high infecting efficiencyof entomopathogenetic nematodes, is safe to people and livestock, natural enemy and environment, and the like, further, breaks the defect that conventional biopesticides cannot prevent and control boring insects, can substitute for high-toxic high-residue chemical pesticides, and has a great significance in ecological protection.

Description

technical field [0001] The invention relates to a method for controlling rice stem borer by using entomopathogenic nematodes, and belongs to the field of biological control of agricultural pests. Background technique [0002] Chilo borer is an important borer pest of rice, which is widely distributed in various rice production areas in my country. In recent years, the occurrence of Chilo borer has a tendency to increase, which poses a serious threat to the safe production of rice. At present, chemical control is still the main method for the larvae of Chilo borer. The large-scale use of chemical pesticides has not only caused non-point source pollution of pesticides, but also caused pesticide residues in agricultural products to exceed the standard, and the resistance of pests to pesticides has increased significantly. Due to the harm of rice borer in the larval stage, the widely used biopesticide Bacillus thuringiensis is not ideal, and it is urgent to develop other high-...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P7/04A01G13/00A01G22/22
CPCA01G13/00A01G22/22A01N63/10
Inventor 韩光杰徐健李传明刘琴祁建杭徐彬陆玉荣
Owner JIANGSU LIXIAHE REGION AGRI RES INST