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Hippocampal mRNA sequence analysis for schizophrenia mouse model and kit

A schizophrenia, mouse model technology, applied in DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve problems such as difficulty in collection, and achieve the effect of improving standardization and success rate

Inactive Publication Date: 2019-10-08
中国人民解放军联勤保障部队第九0四医院
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Problems solved by technology

However, subject to the limitations of bioethics, it is difficult to collect living brain tissue based on human schizophrenia. Recently, the neurodevelopmental mouse model of schizophrenia has attracted the attention of researchers. At present, there are mature theoretical foundations and modeling standards. and detection indicators, which have obvious advantages in mechanism validity, epigenetic validity and prediction validity

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  • Hippocampal mRNA sequence analysis for schizophrenia mouse model and kit
  • Hippocampal mRNA sequence analysis for schizophrenia mouse model and kit
  • Hippocampal mRNA sequence analysis for schizophrenia mouse model and kit

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Embodiment Construction

[0032] A hippocampal biomarker of a neurodevelopmental mouse model of schizophrenia, wherein the markers are mRNA1, mRNA2, mRNA5, and mRNA10.

[0033] A method for detecting hippocampal biomarkers in a neurodevelopmental mouse model of schizophrenia, comprising the following steps:

[0034] Brain tissue collection, three groups of mice in the control group (NC), schizophrenia model group (SZ), and treatment group (Treated) were decapitated at 100 days and the whole brain was removed;

[0035] RNA extraction and library construction to extract the total RNA of the sample, and use the kit ribo-zero to digest the ribosomal RNA; add the interrupting reagent to interrupt the RNA into short fragments, use the interrupted RNA as a template, and use six base random Primers are used to synthesize one-strand cDNA, and then prepare a two-strand synthesis reaction system to synthesize two-strand cDNA. During cDNA second-strand synthesis, dUTP is used instead of dTTP, and then different ad...

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Abstract

The invention belongs to the technical field of biology, and relates to a hippocampal biomarker for a schizophrenia neurodevelopment mouse model, a detection method and a kit. The invention provides:1) the hippocampal biomarker for a neurodevelopmental mouse model of schizophrenia, which is mRNA1, mRNA2, mRNA5, mRNA10, the detection method for the above-mentioned marker, and the detection kit; 2)the biomarker for hippocampal intervention of the schizophrenia neurodevelopment mouse model, which is mRNA10, the detection method for the above marker, and the detection kit; and 3) mRNA10 mRNA signaling pathway-target gene network map and GO / KEGG enrichment analysis results. The beneficial effects of the present invention are that the schizophrenia neurodevelopment mouse model and the biomarker of intervention are provided for the first time, and the hippocampal mRNA has the difference in expression level. The change can be reversed by olanzapine intervention, which has implications for revealing the pathogenesis of schizophrenia and the therapeutic targets of schizophrenia.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a schizophrenia mouse model hippocampal mRNA sequence analysis and kit. Background technique [0002] Schizophrenia (SZ) is a severe mental illness, affecting 1% of the world's population, with a lifetime prevalence of 0.3%-0.7%, 5%-6% of patients eventually die by suicide, and 20% of patients With suicidal ideation or behavior, patients may have hallucinations, delusions, emotional apathy, social withdrawal and other typical mental symptoms, loss or incomplete insight, resulting in severe cognitive, emotional, behavioral and other psychosocial function damage; the disease may be concealed Onset, insidious course, poor prognosis, recurrent attacks, mainly in young adults, and large inter-individual variability in symptom manifestations and prognosis. [0003] According to the existing research, genetics plays an important role in the pathological mechanism and pathological process o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/118C12Q2600/158C12Q2600/166
Inventor 张理义
Owner 中国人民解放军联勤保障部队第九0四医院
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