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Closed sequencing library preparation card box

A sequencing library and closed technology, applied in biochemical equipment and methods, microbial measurement/inspection, biochemical instruments, etc., can solve the problems of sample environmental pollution, uncontrollable results, errors, etc., to avoid experimental errors and improve operation Efficiency and the effect of reducing the probability of errors

Pending Publication Date: 2019-10-15
上海思路迪医学检验所有限公司
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Problems solved by technology

The disadvantage of this operation is that the probability of human error is very high, the result is uncontrollable, and the samples are very easy to pollute the environment, and the risk of cross-contamination between samples is greatly increased

Method used

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  • Closed sequencing library preparation card box
  • Closed sequencing library preparation card box
  • Closed sequencing library preparation card box

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Embodiment Construction

[0035] While the present invention will be fully described with reference to the accompanying drawings containing preferred embodiments of the invention, it should be understood before proceeding that those skilled in the art may modify the invention described herein while obtaining the technical effects of the present invention. Therefore, it should be understood that the above description is a broad disclosure for those skilled in the art, and its content is not intended to limit the described exemplary embodiments of the present invention.

[0036] In addition, in the following detailed description, for purposes of explanation, numerous specific details are set forth in order to provide a comprehensive understanding of the embodiments of the present disclosure. It may be evident, however, that one or more embodiments may be practiced without these specific details. In other instances, well-known structures and devices are shown in diagrammatic form to simplify the drawings....

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Abstract

The invention discloses a closed sequencing library preparation card box including a shell, a circular base plate and an injection unit; the shell is internally provided with a shaft extending downwards from the top wall of the shell; the circular base plate is provided with a plurality of accommodating grooves distributed at intervals in the circumference; the center of the circular base plate isprovided with a through hole for the shaft to pass through, so that the circular base plate can rotate in the shell around the shaft; the injection unit is located in the shell, and includes an injection main body connected with the shell and an injection head in fluid communication with the injection main body. The shell is provided with a first orifice for a driving mechanism to enter so as tooperate the injection main body, and the injection head can carry out lifting movement in the shell to inject and suck fluid of the accommodating grooves under the action of the injection main body. By replacing manual operation with the injection unit, the card box can greatly improve the operation efficiency, reduce the probability of occurrence of error and avoid the experimental error caused by artificial reagent addition and the potential risk generated to an operator.

Description

technical field [0001] The disclosure belongs to the technical field of gene sequencing, and in particular relates to a closed sequencing library preparation cartridge. Background technique [0002] After the 1950s, with the development of molecular genetics, people gradually realized that the essence of genes is DNA fragments that control biological traits and have genetic effects. On the one hand, genes can faithfully replicate themselves to ensure the basic characteristics of organisms; on the other hand, genes can also mutate, leading to diseases. Gene sequencing can quickly and accurately obtain biological genetic information, reveal the complexity and diversity of the genome, and play a very important role in life science research; similarly, gene sequencing is also of great significance and value to clinical medicine. Mechanism research and clinical diagnosis gradually rely on gene sequencing technology. [0003] The current main method of gene sequencing library pr...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12Q1/6806
Inventor 谢世飞陈才夫华锦彪周建涛何开伟熊磊
Owner 上海思路迪医学检验所有限公司
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