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A particle separation, low-speed technology, used in centrifuges with rotating drums, centrifuges, etc., can solve problems such as easy failure and complex process
Active Publication Date: 2019-10-18
安徽益必生物科技有限公司
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[0003] Although ultracentrifugation can solve the problem of recovery of biological or non-biological microscopic particles suspended in liquid to a certain extent, the sample preparation process and experimental operation of ultracentrifugation, especially the process of density gradient centrifugation are very complicated and prone to failure. This case provides A new separation method was developed to solve this technical problem
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Embodiment 1
[0024] Embodiment 1, see Figure 1-2 , the present invention provides a technical solution: a low-speed micro-particle separation device, including a motor 1, a transmission shaft 2, a sample separation cup 3, a liquid inlet pipe 4, a sample collection tank 5, a liquid discharge pipe 6 and a sealing isolation layer 7 , the motor 1 is connected to the sample separation cup 3 through the transmission shaft 2, and the sample separation cup 3 is separated from the motor 1 by a sealing isolation layer 7, and the periphery of the cup opening of the sample separation cup 3 is provided with an annular A sample collection tank 5, the bottom of the sample collection tank 5 is provided with one or several funnel-shaped openings, and is connected to a liquid discharge pipe 6, and the inner cavity of the sample separation cup 3 is provided with a liquid inlet pipe 4 along the up and down direction;
[0025] The sealing isolation layer 7 can be extended as a housing or a flat plate that wra...
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Abstract
The invention discloses a low-rotation-speed micro-particle separation device. According to the design, the centrifugal force is used for overcoming self-gravity of the liquid, so that the centrifugalforce can climb upwards from bottom to top in the sample separation cup, a thin layer is formed on the inner wall, a special limiting space is formed by the liquid thin layer, micro particles are subjected to polarization brownian motion, differential migration is realized, the aim of separating the micro particles is achieved, the device comprises a motor, a transmission shaft, a sample separation cup, a liquid inlet pipe, a sample collection tank, a liquid discharge pipe and a sealing isolation layer, wherein the motor is connected with the sample separation cup through the transmission shaft. The method can be used in the industrial field, such as production of colloidal gold particles, diamond sorting for cutting, high-grade coal, mineral refining and the like, and can also be used inthe field of biological medical treatment, and microorganisms, the method can be used for separating and analyzing different types of microorganisms, and is simple in separation means, high in success rate, wide in application range and high in practicability.
Description
technical field [0001] The invention relates to the technical field of micro-particle separation, in particular to a low-speed micro-particle separation device. Background technique [0002] The size of chemical molecules reaches the range of colloidal particles, which can form a stable suspension (that is, colloidal solution); even if some biological or non-biological particles are slightly larger than the size of colloidal particles, the suspension is relatively stable and needs to stand for a long time Time can settle down. In order to quickly separate tiny particles like proteins from quasi-stable suspensions, Swedish scholar Theodor Svedberg invented the ultracentrifuge in the 1920s. The purpose of his original invention was not only to separate proteins, but also to It is important to measure protein molecular weight. In any case, ultracentrifugation has become a classic means of separating colloidal particles and other biological and non-biological tiny particles. Th...
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