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Enterobacter hy1 and its application in degrading bishydroxyethyl terephthalate

A technology of bishydroxyethyl phthalate and Enterobacteriaceae, which is applied in the field of Enterobacteria HY1 and in the degradation of bishydroxyethyl terephthalate, which can solve the problems of long existence period and pollution of organic matter

Active Publication Date: 2021-06-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Polyethylene terephthalate (PET) is one of the most widely used organic compounds. PET waste after a large amount of use is highly resistant to environmental microbial degradation, resulting in a long life cycle. PET waste has become a serious global environmental pollution organic matter

Method used

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  • Enterobacter hy1 and its application in degrading bishydroxyethyl terephthalate

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Experimental program
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Embodiment 1

[0034] 1. Materials and methods

[0035] 1.1 Medium:

[0036] (1) Basic inorganic salt medium (BSM): K 2 HPO 4 ·3H 2 O 1.0g / L, NaCl 1.0g / L, (NH 4 ) 2 SO 4 0.5g / L, MgSO 4 ·7H 2 O 0.4g / L, CaCl 2 0.0755g / L, FeCl 3 ·6H 2 O 0.0143g / L, the solvent is distilled water, pH 7.5, sterilized at 115°C for 30min.

[0037] (2) LB medium: peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, solvent is distilled water, pH 7.0, sterilized at 115°C for 20min.

[0038] (3) Screening culture medium: 10 g / L of BHET in DMSO solution was added to the BSM medium, so that the final concentration of BHET in the medium was 250 mg / L.

[0039] The solid selection medium is to add 15g / L agar to the selection medium.

[0040] 1.2 Reagents: Bis(2-hydroxyethyl) terephthalate (BHET), analytically pure, purchased from Sinopharm Chemical Reagent Co., Ltd.; Methanol (HPLC grade) was purchased from Tianjin Siyou Fine Chemicals Co., Ltd.; other reagents All are analytically pure.

[0041] 1.3 Method

[00...

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Abstract

The invention discloses an Enterobacter HY1 and its application in degrading bishydroxyethyl terephthalate. Enterobacter HY1 is used as an enzyme source, bishydroxyethyl terephthalate is used as a substrate, and basic inorganic salts are used for cultivation. The base is used as the reaction medium, reacting at 25-37°C to achieve the degradation of the substrate; the new strain-Enterobacter sp.HY1 with the ability to degrade bishydroxyethyl terephthalate, the optimum temperature and optimum The optimal pH values ​​were 37℃ and 8.0, and the optimal nitrogen source was yeast extract. The degradation rates of 250, 500, and 1000 mg / L BHET within 120 hours were 93.46%, 87.73%, and 80.81%, respectively.

Description

[0001] (1) Technical field [0002] The invention relates to a bishydroxyethyl terephthalate (BHET) degrading bacterium—Enterobacter sp. HY1 and its application in microbial degradation of bishydroxyethyl terephthalate (BHET). [0003] (2) Background technology [0004] Polyethylene terephthalate (PET) is one of the most widely used organic compounds. PET waste after a large amount of use is highly resistant to environmental microbial degradation, resulting in a long life cycle. PET waste has become a serious global environmental pollution organic matter. Bishydroxyethyl terephthalate (BHET) can be produced by esterification of terephthalic acid (TA) and ethylene glycol (EG). BHET is one of the intermediates produced during the synthesis and degradation of PET. Its chemical structure unit is similar to that of PET. It is one of the most suitable model compounds for studying the biodegradation of PET. In addition, BHET can be volatilized into the atmosphere due to the incinera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/72C12R1/01C02F101/34
CPCB01D53/72B01D53/84C02F3/34C02F2101/34C12N1/205C12R2001/01Y02A50/20
Inventor 邱乐泉吴石金钟卫鸿张汉虞刘腾飞尹辛格
Owner ZHEJIANG UNIV OF TECH
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