Fluorescent affinity immunochromatographic detection kit for osylated hemoglobin and detection method thereof
An immunochromatographic detection and glycated hemoglobin technology, which is applied in the field of glycated hemoglobin detection, can solve the problem that the glycated hemoglobin detection cannot meet the requirements of rapid diagnosis and accuracy at the same time, and achieves the effects of good performance, good accuracy and good consistency
Pending Publication Date: 2019-10-18
NANJING NANOEAST BIOTECH
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[0011] In view of the shortcomings of the above-mentioned existing detection technologies, the purpose of the present invention is to provide a fluorescent affinity immunochromatographic detection kit for glycated hemoglobin, which aims to solve the problem that the detection of glycated hemoglobin in the prior art cannot meet the requirements of rapid diagnosis and accuracy at the same time. question
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[0030] The glycosylated hemoglobin fluorescence affinity immunochromatography detection kit provided by the invention. In order to make the above-mentioned features and advantages of the present invention more comprehensible, the following specific implementation examples are described in detail with the accompanying schematic diagrams, wherein the same reference numerals indicate the same or similar units or steps. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0031] figure 1 It is the component of the glycated hemoglobin fluorescence affinity immunochromatography detection kit provided in the embodiment of the present invention. Such as figure 1 As shown, the glycated hemoglobin fluorescence affinity immunochromatography detection kit includes: a diluent 101, a fluorescent immune microsphere solution 102, and a fluorescent affinity immunochromatography test strip. ...
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Abstract
The invention discloses a fluorescent affinity immunochromatographic detection kit for osylated hemoglobin and a detection method thereof. The kit comprises a diluent, a fluorescent immunosphere solution, and a fluorescent afinity immunochromatographic test strip. The diluent is used as a buffer solution for hemolysis, incubation, and dropping. The fluorescent immunosphere solution is a label carrier for providing an accurate and sensitive fluorescent signal. The fluorescent afinity immunochromatographic test strip is a lateral chromatographic test strip including a bottom plate, a sample pad,a nitrocellulose membrane with a boric acid affinity chromatography zone and a quality control line, and absorbent paper. Besides, the fluorescent affinity immunochromatographic detection kit detection method includes a hemolysis method, a fluorescent immunomicrosphere incubation method and a drop sample detection method. The disclosed kit has advantages of the affinity chromatography method andthe fluorescence immunochromatography method and is capable of detecting the glycosylated hemoglobin accurately and sensitively.
Description
technical field [0001] The invention relates to the technical field of glycosylated hemoglobin detection, in particular to a glycosylated hemoglobin fluorescence affinity immunochromatography detection kit and detection method. Background technique [0002] Glycated hemoglobin (HbA1c) is precisely defined as a stable compound of glucose and the N-terminal valine residue of the beta chain of hemoglobin. In the body, once hemoglobin contacts with glucose, non-enzymatic glycosylation can occur, and the degree of glycosylation depends on the contact time of protein and sugar and the concentration of sugar. In the initial stage, an unstable Schiff base (precursor of HbA1c) is formed, and the precursor of glycosylated hemoglobin undergoes Amadori rearrangement to form HbA1c. The formation process of this ketone structure is slow and irreversible, and runs through the average lifespan of red blood cells. It is worth mentioning that the precursor of glycosylated hemoglobin always e...
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IPC IPC(8): G01N33/72
CPCG01N33/723G01N33/726
Inventor 张宇王建国韩国志
Owner NANJING NANOEAST BIOTECH