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Preparation method and application of cichorium glandulosum Boiss lignin extract

A technology of lignin and extract, which is applied in the field of biomedicine, can solve the problems of lack of anti-inflammatory effect of non-water-soluble components, limitation of the use of chicory water extract, and difficulties in pharmacological and toxicological research of the mixture

Active Publication Date: 2019-10-22
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the anti-inflammatory effect of the original medicinal material Chicory is not obvious, and it is necessary to separate and purify components with anti-inflammatory effects from Chicory in order to better exert the anti-inflammatory effect of Chicory
The research on the anti-inflammatory components of chicory hair mainly focuses on the water extract of chicory hair (experimental research on the anti-inflammatory effect of water extract of hairy chicory, Yang Qiaoli, China Medical Herald, 2016), which revealed that the water extract of hair chicory inhibited The molecular mechanism of inflammation, but there are still the following problems: (1) the water extract of Chicory hairy is a mixture of various water-soluble components, and there is no single compound that has the effect of inhibiting inflammation in Chicory hairy. Toxicological research is relatively difficult, thereby limiting the clinical application of Chicory hairy water extract; (2) Chicory hairy also contains a large amount of non-water-soluble components, and the non-water-soluble components also have anti-inflammatory effects on the original drug Chicory hairy. Certain contribution, but the scheme of the prior art lacks anti-inflammatory effect research on non-water-soluble components

Method used

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  • Preparation method and application of cichorium glandulosum Boiss lignin extract
  • Preparation method and application of cichorium glandulosum Boiss lignin extract
  • Preparation method and application of cichorium glandulosum Boiss lignin extract

Examples

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Effect test

Embodiment 1

[0049] Example 1: C 20 h 24 o 6 The preparation process

[0050] Step (1) pretreatment: drying the chicory root in the air, and making the moisture content of the chicory root 5%.

[0051] Step (2) Alcoholic cold extraction: adopt cold extraction method, get the dry chicory root 5kg, soak 5 times with 4 times the amount of 95% ethanol, each time for 3 days, collect the filtrate soaked every time, and mix 5 times The collected filtrates were combined.

[0052] Step (3) Concentration: Take the combined filtrate, use a vacuum distillation device, under the conditions of a pressure of 0.1 MPa and a temperature of 45° C., to remove ethanol by distillation to obtain a thick extract of 0.3 g / ml.

[0053] Step (4) extraction and purification: disperse 1 volume part of the thick extract into 3 volume parts of water to obtain a suspension; use 1 volume part of n-butanol to extract 1 volume part of the suspension, and take the water layer; 1 part by volume of the aqueous layer was e...

Embodiment 2

[0062] Example 2: C 20 h 24 o 6 The preparation process

[0063] The present embodiment is basically the same as Example 1, the difference being that in the step (2) alcohol cold extraction, the cold extraction method is used, and the dried chicory root is taken and soaked 5 times with 3 times the amount of 95% ethanol. For the next 3 days, collect the filtrate of each immersion, and combine the filtrate collected for 5 times. The silica gel type of silica gel column I, silica gel column II and silica gel column III are all silica gel HF.

Embodiment 3

[0064] Example 3: C 20 h 24 o 6 The preparation process

[0065] The present embodiment is basically the same as Example 1, the difference being that: in the step (2) alcohol cold extraction, adopt the cold extraction method, take the dried chicory root, soak 5 times with 5 times the amount of 95% ethanol, each time For the next 3 days, collect the filtrate of each immersion, and combine the filtrate collected for 5 times. The silica gel type of silica gel column I, silica gel column II and silica gel column III is silica gel G.

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Abstract

The invention belongs to the field of biological medicine, and relates to a preparation method and application of a cichorium glandulosum Boiss lignin extract, in particular to the preparation methodand application of the cichorium glandulosum Boiss lignin extract. The cichorium glandulosum Boiss lignin extract C20H24O6 can be taken as an inflammation lead compound, the compound has an inhibitioneffect on NO, TNF-alpha and PGE2 which are generated from RAW264.7 cells activated by LPS, is low in cytotoxicity and can significantly reduce expression of COX-2 protein; the compound can also improve the increase phenomenon, caused by the LPS, of the mouse spleen index and thymus index and reduce the concentration of IL-1beta, caused by the LPS, in serum of a mouse. According to the technical scheme, the novel application of the lignin compound C20H24O6 and the preparation method of the compound are provided, and according to the novel application and preparation method of the compound, theapplication range of the compound can be enlarged. According to the technical scheme, the compound can be applied to the fields of medicine preparation and the like.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a preparation method and application of a chicory extract, in particular to a preparation method and an application of a chicory lignin extract. Background technique [0002] Chicory (Cichorium glandulosum Boiss) is a biennial or perennial perennial plant of the genus Cichorium in the family Asteraceae. It is native to the Mediterranean, Central Asia and North Africa, and is widely cultivated in Europe. There is a record in the national standard of medicinal materials "Uyghur Medicine Chronicle": "Chicory can be used as medicine. It tastes slightly bitter, salty, and cool in nature. Uyghur medicine is widely used in the treatment of liver and gallbladder diseases such as hepatitis and cholecystitis." [0003] However, the anti-inflammatory effect of the original medicinal material Chicory is not obvious, and it is necessary to separate and purify components with anti-inflammatory effects...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61P29/00C07D307/80
CPCA61K31/343A61P29/00C07D307/80
Inventor 秦冬梅邹楠袁今奇陈卫军潘馨慧韩畅苏林洁党婷
Owner SHIHEZI UNIVERSITY
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