A kind of effective part of Dendrobium melon and its preparation method and application

An effective part, the technology of Dendrobium melon, is applied in the direction of medical preparations, applications, and pharmaceutical formulas containing active ingredients, which can solve the problem that the effective part and composition of the extract cannot be clarified, and achieve strong inhibition of α-glucosidase activity , strong antioxidant capacity, strong effect on HT22 cell damage

Active Publication Date: 2021-11-16
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the extracts of Dendrobium melons with hepatoprotective effects have been reported in the prior art, these extracts have failed to clarify the effective parts and components.

Method used

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  • A kind of effective part of Dendrobium melon and its preparation method and application
  • A kind of effective part of Dendrobium melon and its preparation method and application
  • A kind of effective part of Dendrobium melon and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 - Preparation of effective parts of Dendrobium melon

[0041] S1. Soak 1 kg of Dendrobium melons with 80% acetone aqueous solution at room temperature, the ratio of solid to liquid is 8:1, ultrasonically assisted extraction, 80min / d, place for 48h, filter, concentrate the filtrate, and obtain the extract of Dendrobium melons; repeat the extraction for 3 Second-rate.

[0042] S2. Suspend the Dendrobium melon extract obtained in S1 in 800mL of water, add an equal volume of ethyl acetate for liquid-liquid extraction, collect the water phase, and spin dry to obtain the effective part of Dendrobium melon. The ethyl acetate extraction was repeated 3 times.

[0043]The results showed that the content of glycoside compounds in the effective parts of Dendrobium melons accounted for 12% of the total mass of the effective parts of Dendrobium melons. The flickinflimoside B content accounts for 7% of the total mass of the effective parts of Dendrobium melon, the conten...

Embodiment 2

[0046] Example 2 - Preparation of effective parts of Dendrobium melon

[0047] S1. Soak 1kg of Dendrobium melons with 80% methanol aqueous solution at room temperature, the ratio of solid to liquid is 10:1, ultrasonically assisted extraction, 80 min / d, place for 48 hours, filter, concentrate the filtrate, and obtain the extract of Dendrobium melons; repeat the extraction 3 times.

[0048] S2. Add the Dendrobium melon extract obtained in S1, add an appropriate amount of methanol to dissolve, and perform solid phase extraction on a C-18 reverse-phase silica gel column. The weight ratio of the dry extract of the Dendrobium melon extract to the C-18 reverse-phase silica gel column stationary phase bed volume ratio 0.3kg: 1L, after loading the sample, remove impurities by washing with 5 times the bed volume of water, then wash with 6 times of 30% methanol-water solution, collect the methanol-water eluate, concentrate and dry to obtain the effective parts.

[0049] The content o...

Embodiment 3

[0050] Example 3 - Preparation of effective parts of Dendrobium melon

[0051] S1. Cold-soak 1 kg of Dendrobium melons with 80% ethanol aqueous solution at room temperature, the ratio of solid to liquid is 10:1, ultrasonically assisted extraction, 80 min / d, place for 48 hours, filter, concentrate the filtrate to obtain the extract of Dendrobium melons; repeat Extract 3 times.

[0052] S2. Add the Dendrobium melon extract obtained in S1, add an appropriate amount of water to dissolve, and perform solid-phase extraction on a macroporous adsorption resin column. The weight ratio of the dry extract of the Dendrobium melon extract to the stationary phase bed of the macroporous resin adsorption resin column is 0.3 kg: 1L, after loading the sample, wash with 5 times the bed volume of water to remove impurities, then wash with 6 times of 40% ethanol-water solution, collect the ethanol-water eluate, concentrate and dry to obtain the effective part of Dendrobium officinale;

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Abstract

The invention relates to the technical field of traditional Chinese medicines, and specifically discloses an effective fraction of Dendrobium melon, a preparation method and application thereof. The main secondary metabolite compound type of the effective part of Dendrobium melon is glycoside compound. The main glycoside components of the effective part of Dendrobium officinalis are pimarane-type monoglycoside, flickinflimoside B, diglycoside fliflidioside A, (+)-syringaresin-O-β-D-glucopyranoside and the like. In vitro activity studies show that the effective fraction of Dendrobium melonii of the present invention has good DPPH free radical and OH free radical scavenging activity, α-glucosidase inhibitory activity, and inhibitory activity of glutamate-induced HT22 cell damage. The effective part of Dendrobium candidum can be developed into an auxiliary drug or health care product for treating Alzheimer's disease, liver protection, type II diabetes and other diseases.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a Dendrobium melon (Dendrobium officinalis) Flickingeria fimbriata ) effective part and its preparation method and application. Background technique [0002] Dendrobium with melon, that is, golden Dendrobium with tassels ( Flickingeria fimbriata ) for Orchid ( Orchidaceae ) Dendrobium ( Flickingeria ) plants, mainly produced in Guangdong (Meizhou area), Guangxi, Hainan, Yunnan, Guizhou and other places. The traditional Chinese medicine Dendrobium candidum is Dendrobium candidum ( Flickingeria fimbriata ) dry whole plant with pseudobulbs, also known as Dendrobium with Melon. The traditional Chinese medicine Dendrobium officinale is recorded in the "Guangdong Provincial Standards of Chinese Medicinal Materials". It tastes sweet and slightly cold. It returns to the stomach, lung and kidney meridians. It is used for yin deficiency and body fluid deficienc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/898A61P25/28A61P1/16A61P3/10A23L33/105A23L33/125A61K31/7028A61K31/7048A61K31/704
CPCA23V2002/00A61K31/7028A61K31/704A61K31/7048A61K36/898A61K2236/333A61K2236/39A61K2236/55A23L33/105A23L33/125A61P1/16A61P3/10A61P25/28A23V2200/30A23V2200/322A23V2200/328A23V2250/21
Inventor 王军李雪文刘岚陈慧萍
Owner SUN YAT SEN UNIV
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