Ultra-high resolution nuclear magnetic resonance two-dimensional J decomposition spectrum method

Abstract

The invention provides an ultra-high resolution nuclear magnetic resonance phase-sensitive two-dimensional J decomposition spectrum method used for acquiring molecular structure information and accurately measuring a J coupling constant. The method is based on a combined action of a ZS decoupling module and an echo chain sampling module, shortcomings of an existing two-dimensional J-spectrum technology in practical applications are overcome, and a high-resolution two-dimensional phase-sensitive J-decomposition spectrum can be acquired under an uneven magnetic field environment. . By using themethod, a spectral resolution of the two-dimensional J decomposition spectrum is increased. The method is of great significance for structure detection of a complex sample of a crowded spectrum peak,and an application field of the two-dimensional J decomposition spectrum is expanded. And simultaneously, based on a monochromatic pulse excitation method, the invention provides a sensitivity enhancement method based on multi-color pulse excitation sampling realiztion.

Description

technical field [0001] The present invention relates to nuclear magnetic resonance (Nuclear Magnetic Resonance, NMR) spectroscopy molecular structure detection technology, in particular to a general method for obtaining ultra-high resolution two-dimensional phase-sensitive J-decomposition spectrum, and to accurately obtain molecular structure in an inhomogeneous magnetic field environment method of information. Background technique [0002] As a robust non-invasive detection technique, NMR spectroscopy has a wide range of applications in disciplines such as chemistry, biology, and life sciences. In particular, two-dimensional J-decomposition spectroscopy, as one of the most important NMR techniques, provides more molecular structure information by displaying chemical shift and J-coupling information in two independent dimensions, and is therefore widely used in Assignment of chemical components and measurement of J coupling constants. The most commonly used method in obtai...

AI Technical Summary

Problems solved by technology

However, in practical applications, this technology has two main limitations

First, due to the phase modulation in the traditional two-dimensional J decomposition spectrum experiment, the two-dimensional J decomposition spectrum obtained by this technique is usually displayed in absolute value mode, which reduces the resolution of the spectrum

This resolution problem further makes it difficult for the traditional two-dimensional J-decomposition method to be applied to the detection of complex samples with crowded peaks.

In particular, for the detection of samples containing strong coupling systems, traditional two-dimensional J-decomposition spectra often show strong coupling artifacts, which brings difficulties in spectrum assignment

Second, the acquisition of the traditional two-dimensional J-decomposition spectrum often puts forward higher requirements on the uniformity of the magnetic field, and usually requires a uniform magnetic field environment to obtain a satisfactory two-dimensional J-decomposition spectrum

For the detection of heterogeneous samples such as viscous samples and biological tissue samples, the inhomogeneous magnetic field caused by the inhomogeneous magnetic susceptibility of the sample usually conceals all useful structural information, and the inherent magnetic susceptibility of this sample Inhomogeneities are often difficult to remove with shimming

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