Lime brew, its preparation method and its medicinal application
A lime, high-performance liquid chromatography technology, applied in pharmaceutical formulations, antipyretics, drug combinations, etc., can solve problems such as lack of overall applicability, short production period, and difficult storage.
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[0027] The preparation method of the present invention comprises: obtaining a substrate comprising a sugar of acidic rough and 10 to 20 weight percent concentrations; wherein the weight percentage concentration is based on the total weight of the matrix; and the matrix Co-cultivation with yeast and root austerus to obtain the orange brewed.
[0028] In one embodiment, "rough" as described in the present invention refers to the residual structure of the hydrate of the whole fruit, which can comprise a peel, an endometrium, and a pulp. In a preferred embodiment, the "residue" of the present invention does not comprise seeds in the fruit. In one embodiment, the method of the present invention further comprises the step of seedling to achieve full fruitless rag. In a preferred embodiment, the orange has a sugar acid ratio of 1.5 to 2.
[0029] In one embodiment, the orange rough is placed in 40 to 80 ° C for 40 to 60 hours before mixing of the saccharide, to perform an aging reaction....
Embodiment 1
[0042] Example 1: Preparation of a yogodilynted cast of the present invention.
[0043] Take the approximately 4.5 kg of fresh tattrates (this experiment is determined with a sugar acid ratio: 1.86). After washing it twice, it is broken, and the whole fruit is broken, and the plant juice equipment is subjected to squeezing . Re-uses the seedling machine to remove the remaining seed seeds in the rumor after the juice, thereby obtaining about 1 kg without seed. Then the obtained ragiza seal was stored in the culture chamber, reacted at 60 ° C for 48 hours (aging reaction). Then, the residue was further dehumidized for 4 hours at 50 ° C; then the above-mentioned semi-drying rosacea will be used in the hot air 50 ° C, frequency 40.68 MHz, and the temperature of 130 cm in the center of the center. Processing 120 seconds, performing sterilization steps. According to the test results, the treated residue has fewer total bacteria and mold number (Table 1) compared to the residue of the ag...
Embodiment 2
[0047] Example 2: The total polyphenol content, total flavonoid content and anti-inflammatory effects of the acidic flavonoids were detected.
[0048] The total polyphenol content and total flavonoid content contained in the tangerine brewing of this experiment were tested. The detection methods used in this experiment are roughly:
[0049] Analysis of total polyphenol compounds: Take 1 mL of 0.2n flan reagent, sequentially add 0.2 ml of 25 to 200 ppm to the test tube or no food acid and 0.8 mL of 7.5% sodium carbonate solution, after uniform oscillating, at room temperature For 30 minutes, the light-absorbance value changes under the wavelength of 765 nm. The concentration of the sample to be tested is converted to the concentration of the neoadjuble acid.
[0050] Total flavonoid content analysis: Retrieving solution (100 mL), adding 30 ml of 5% nitrite for 6 minutes. Then, 5% aluminum nitrate was added for 6 minutes, then 100 ml of 1 M sodium hydroxide was added, and the light-...
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