Staphylococcus culture solution and culture method thereof

Abstract

The invention discloses a staphylococcus culture solution and a culture method thereof. The staphylococcus culture solution is prepared from the components by weight: 1000 ml of pig heart filtrate, 10g of peptone, 3 g of a beef extract, 5 g of yeast powder, 15 g of sodium chloride, 0.02 g of potassium tellurite, 5 g of sodium pyruvate, 5 g of lithium chloride, 30 g of sugar and 10 g of activatedcarbon. The culture method comprises the steps: the pig heart filtrate is prepared; the peptone and the beef extract are added into the prepared pig heart filtrate in twice, the yeast powder, the sodium chloride, the potassium tellurite, the sodium pyruvate, the lithium chloride and the sugar are added under certain conditions, the pH is adjusted in twice, and still standing overnight and filtering are conducted separately; then the activated carbon is added, then filtering is conducted to remove the activated carbon, and then whey is added to make a pig heart culture medium; and a seed culture medium is inoculated into the pig heart culture medium to be cultured. According to the staphylococcus culture solution and the culture method thereof, interference of bacteria is inhibited througha manufactured salty environment, and meanwhile the yield of staphylococcus is greatly increased by adding auxiliary materials.

Description

technical field [0001] The invention belongs to the technical field of staphylococcus culture, in particular to a staphylococcus culture solution and a culture method thereof. Background technique [0002] Coccus refers to a spherical or nearly spherical bacteria; according to different arrangements, it can be divided into monococcus, diplococcus, streptococcus, tetrad, sarcina and staphylococcus. Staphylococcus is a group of Gram-positive staphylococci, which are often piled up into bunches of grapes, hence the name. Most are non-pathogenic bacteria, a few can cause disease. Staphylococcus is the most common Staphylococcus pyogenes, which is an important source of cross-infection in hospitals. The diameter of the bacteria is about 0.8 μm, small spherical, but in the young culture of liquid medium, it is often dispersed, and the bacterial cells exist alone; has important research significance. [0003] Staphylococci need to be cultured before research, and the culture med...

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Problems solved by technology

[0003] Staphylococci need to be cultured before research, and the culture medium is an important part of cultivating staphylococci; existing staphylococci are prone to produce miscellaneous bacteria during cultivation, and the existence of miscellaneous bacteria will greatly interfere with the later research of staphylococci; in addition Existing staphylococcus culture yields are low

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