Application of pepperweed seed and Chinese date lung purging soup adopting golden chamber synopsis formula in preparation of drug for inhibiting esophageal cancer cells

A kind of water extract, esophageal cancer technology

Active Publication Date: 2019-11-01
HENAN UNIV OF CHINESE MEDICINE
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  • Application of pepperweed seed and Chinese date lung purging soup adopting golden chamber synopsis formula in preparation of drug for inhibiting esophageal cancer cells
  • Application of pepperweed seed and Chinese date lung purging soup adopting golden chamber synopsis formula in preparation of drug for inhibiting esophageal cancer cells
  • Application of pepperweed seed and Chinese date lung purging soup adopting golden chamber synopsis formula in preparation of drug for inhibiting esophageal cancer cells

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[0019] The specific implementation of the present invention will be described in detail below in combination with specific situations.

[0020] Effective prescriptions that can significantly inhibit the activity of four types of esophageal cancer cells (EC9706, Eca109, EC-1, TE-1) were screened from 157 first prescriptions in "Synopsis of the Golden Chamber" by using the MTT method; the effective prescriptions that were screened were observed with an inverted microscope The morphological changes of the four types of esophageal cancer cells were photographed and recorded; the effects of the selected prescriptions on the growth cycle of the four types of esophageal cancer cells were detected and analyzed by flow cytometry; the RTCA real-time unlabeled cell analyzer was used to monitor, study and Analyze the effects of the selected prescriptions on the proliferation, migration, and infiltration of four types of esophageal cancer cells; use soft agar colony formation experiments to...

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Abstract

The invention relates to application of pepperweed seed and Chinese date lung purging soup adopting golden chamber synopsis formula in preparation of a drug for inhibiting esophageal cancer cells andcan effectively solve the problem of preparation of drugs for inhibiting esophageal cancer EG9706, Eca109, EC-1 and TE-1 cells. The pepperweed seed and Chinese date lung purging soup drug is preparedas follows: 10 g of papperweed seeds and 12 Chinese dates are soaked in water with weight 15-20 times of the drug for 1 h, the materials are boiled over strong fire by an electric stove and decocted by soft fire for 1 h, filtration is performed and filtrate is concentrated. The concentrated liquid has effects of significantly inhibiting esophageal cancer EG9706, Eca109, EC-1 and TE-1 cells and iseffectively used for preparing the drug for inhibiting esophageal cancer EG9706, Eca109, EC-1 and TE-1 cells. The raw materials are rich, the preparation method is simple, production and preparation are easy, theoretical and technical support are provided for research of prescriptions for inhibiting migration, proliferation and infiltration of esophageal cancer cells, and the soup has actual clinical significance in treating esophageal cancer.

Description

technical field [0001] The invention relates to medicine, in particular to the application of a water extract of Tingli Dazao Xiefei Decoction, a prescription of Synopsis of the Golden Chamber, in the preparation of medicines for inhibiting esophageal cancer cells. Background technique [0002] Esophageal cancer mainly has two pathological types: esophageal squamous cell carcinoma and adenocarcinoma. According to the 2018 World Cancer Report, Asia is still a high-risk area for esophageal squamous cell carcinoma. In my country, esophageal squamous cell carcinoma is the main type. Long-term smoking, excessive drinking, Eating too fast, overheating, liking to eat pickles, chewing betel lang, psychological factors, family history, eating habits, health awareness, etc. are closely related to the occurrence of esophageal cancer. The main causes of esophageal cancer are summarized as smoking, obesity and acid reflux. Most of the early symptoms of esophageal cancer are not obvious. ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/725A61P35/00
CPCA61K36/31A61K36/725A61K2236/331A61K2236/51A61P35/00A61K2300/00
Inventor 司富春
Owner HENAN UNIV OF CHINESE MEDICINE
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