A kind of artemisia spp. polysaccharide and its extraction method and application
An extraction method and polysaccharide technology, which are applied in the field of biomedicine to achieve the effects of high efficiency, good economic and social benefits, and a simple extraction method
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Embodiment 1
[0023] A method for extracting Artemisia spp. polysaccharides, comprising the following steps:
[0024] (1) Rough extraction; get Artemisia spp., reflux and degrease 2 times with 90% ethanol with a mass percentage concentration, filter to obtain filter residue; add water to extract in the filter residue, wherein the quality of adding water is 6% of the quality of the filter residue times; the number of times of water extraction is 3 times, and the time of each water extraction is 30 minutes; the extraction method is carried out by decoction. Combine and concentrate the obtained extracts, add absolute ethanol, mix well, let stand for alcohol precipitation for 24 hours, collect the precipitate, and dry to obtain the crude extract of Artemisia spp.
[0025] (2) Dissolving the crude extract of Artemisia spp. with water, adding Sevag reagent (chloroform:n-butanol=5:1, V / V) to the solution, mixing, and standing to obtain a deproteinized polysaccharide solution; The volume ratio of ...
Embodiment 2
[0028] A method for extracting Artemisia spp. polysaccharides, comprising the following steps:
[0029] (1) Coarse extraction; get Artemisia spp., reflux and degrease 2 times with 95% ethanol with mass percent concentration, filter to obtain filter residue; add water to extract in the filter residue, wherein the quality of adding water is 8% of the quality of the filter residue times; the number of times of water extraction is 5 times, and the time of each water extraction is 20 minutes; the extraction method is carried out by decoction. Combine and concentrate the obtained extracts, add absolute ethanol, mix well, let stand for alcohol precipitation for 36 hours, collect the precipitate, and dry to obtain the crude extract of Artemisia chinensis;
[0030] (2) Dissolving the crude extract of Artemisia spp. with water, adding Sevag reagent (chloroform:n-butanol=5:1, V / V) to the solution, mixing, and standing to obtain a deproteinized polysaccharide solution; The volume ratio o...
Embodiment 3
[0033] A method for extracting Artemisia spp. polysaccharides, comprising the following steps:
[0034] (1) Coarse extraction; Get Artemisia spp., reflux and degrease 2 times with 70% ethanol with a mass percentage concentration, filter to obtain filter residue; add water to extract in the filter residue, wherein the quality of adding water is 4% of the quality of the filter residue times; the number of times of water extraction is 1 time, and the time of water extraction is 150 minutes; the extraction method is carried out by decoction. Combine and concentrate the obtained extracts, add absolute ethanol, mix evenly, let stand for alcohol precipitation for 12 hours, collect the precipitate, and dry to obtain the crude extract of Artemisia spp.
[0035] (2) Dissolving the crude extract of Artemisia spp. with water, adding Sevag reagent (chloroform:n-butanol=5:1, V / V) to the solution, mixing, and standing to obtain a deproteinized polysaccharide solution; The volume ratio of so...
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