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A compound nby-10 with anti-inflammatory activity extracted from burdock leaves and its preparation method and application

A technology of burdock leaves and compounds, applied in the field of compounds with anti-inflammatory activity, to achieve the effect of rich raw materials, high product purity and strong orientation

Active Publication Date: 2022-06-24
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"The burdock leaf is expected to become a new new resource for inhibiting inflammation, from which safe and effective compounds for inhibiting inflammation can be obtained, and used to prepare anti-inflammatory drugs. There has been no public report so far.

Method used

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  • A compound nby-10 with anti-inflammatory activity extracted from burdock leaves and its preparation method and application
  • A compound nby-10 with anti-inflammatory activity extracted from burdock leaves and its preparation method and application
  • A compound nby-10 with anti-inflammatory activity extracted from burdock leaves and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The present invention is a preparation method of compound NBY-10 with anti-inflammatory activity extracted from burdock leaves, comprising the following steps:

[0028] (1) 20kg of burdock leaf powder, extracted 2 times with 5 times the weight of burdock leaf powder and 30% mass concentration of ethanol, each extraction was 2.5h, the extracts were combined, and concentrated to a concentrated solution equivalent to 0.5g / mL of crude drug;

[0029] (2) The concentrated solution was extracted 4 times with n-butanol 7L, 7L, 4L and 2L in turn, the n-butanol extracts were combined, and the solvent was concentrated under reduced pressure to obtain component Fr.A (60g); component Fr.A was subjected to silica gel Column chromatography, inner diameter 4cm, height 25cm, eluted with a volume ratio of dichloromethane:methanol=100:2 solution 8L, collecting the eluent, and recovering the solvent under reduced pressure to obtain component Fr.A-1 (17g);

[0030] (3) Component Fr.A-1 was ...

Embodiment 2

[0034] The present invention is a preparation method of compound NBY-10 with anti-inflammatory activity extracted from burdock leaves, comprising the following steps:

[0035] (1) 20kg of burdock leaf powder, extract 3 times with 4 times the weight of burdock leaf powder and 30% mass concentration ethanol, extract 3h each time, combine the extracts, and concentrate to a concentrated solution equivalent to 0.6g / mL of crude drug;

[0036] (2) The concentrated solution was extracted 4 times with n-butanol 6L, 6L, 3L and 2L in turn, the n-butanol extracts were combined, and the solvent was concentrated under reduced pressure to obtain component Fr.A (85g); component Fr.A was subjected to silica gel Column chromatography, inner diameter 7cm, height 20cm, eluted with 24L of dichloromethane:methanol=100:2 solution by volume, collected the eluent, and recovered the solvent under reduced pressure to obtain component Fr.A-1 (27g);

[0037] (3) Component Fr.A-1 was subjected to secondary...

Embodiment 3

[0041] The present invention is a preparation method of compound NBY-10 with anti-inflammatory activity extracted from burdock leaves, comprising the following steps:

[0042] (1) 20kg of burdock leaf powder, extracted 2 times with 6 times the weight of burdock leaf powder and 30% mass concentration ethanol, each extraction was 2h, combined the extracts, and concentrated to a concentrated solution equivalent to 0.5g / mL of crude drug;

[0043] (2) The concentrated solution was extracted 4 times with n-butanol 3L, 3L, 4L and 1L in turn, the n-butanol extracts were combined, and the solvent was concentrated under reduced pressure to obtain component Fr.A (75g); component Fr.A was subjected to silica gel Column chromatography, inner diameter 6cm, height 17cm, eluted with a volume ratio of dichloromethane:methanol=100:2 solution 20L, collected the eluent, and recovered the solvent under reduced pressure to obtain component Fr.A-1 (23g);

[0044] (3) Component Fr.A-1 was subjected t...

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Abstract

The invention relates to a compound NBY-10 with anti-inflammatory activity extracted from burdock leaves and its preparation method and application, which can effectively solve the problem of preparing anti-inflammatory compounds from burdock leaves and realize the application of the compound in the preparation of anti-inflammatory drugs The problem, the method is, the burdock leaves are extracted with 30% ethanol, concentrated, the concentrated solution is extracted with n-butanol, the solvent is concentrated under reduced pressure, and silica gel column chromatography is carried out, with dichloromethane-methanol, the solvent is recovered under reduced pressure, and the secondary silica gel column layer Analysis, gradient elution with petroleum ether-ethyl acetate, collecting 100:40 eluate, recovering solvent under reduced pressure, carrying out Sephadex LH-20 column chromatography, eluting with methanol, recovering solvent under reduced pressure, recrystallization in methanol, The compound NBY-10 with anti-inflammatory activity extracted from burdock leaves was obtained. The invention has abundant raw materials, the preparation method is easy to operate, has strong orientation, fast separation speed, high efficiency and high product purity, and the compound can be effectively used for preparing anti-inflammatory drugs.

Description

technical field [0001] The invention relates to medicine, in particular to a compound NBY-10 with anti-inflammatory activity extracted from burdock leaves and a preparation method and application thereof. Background technique [0002] Burdock leaf is the dry basal leaf of Arctium lappa, which is widely distributed throughout the country and has a long history of edible and medicinal use. Modern research shows that burdock leaf is rich in nutrients, rich in inulin, cellulose, carotene, protein, calcium , phosphorus, iron and other minerals and vitamins, have a strong health care function. At the same time, it has anti-oxidative and anti-inflammatory effects, and it is a good tonic for both medicine and food. Numerous herbal works have recorded the therapeutic experience of burdock leaves. For example, "Hygienic Simple Prescriptions" "People who do not cure various sores and golden sores use burdock leaves to paste them." Burdock leaves are expected to become a new new resou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/92A61P29/00
CPCC07D307/92A61P29/00
Inventor 许菲斐刘雅琳龚曼吕江南梁韩晶冯庆梅池军
Owner HENAN UNIV OF CHINESE MEDICINE
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