Novel material and method for promoting plant growth and arsenic-absorbing accumulation

A technology that promotes plant growth and new materials is applied in the field of environmental protection to achieve the effect of promoting arsenic absorption and accumulation and promoting plant growth

Active Publication Date: 2019-11-01
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, aiming at the defects of current heavy metal pollution engineering restoration and phytoremediation, the present invention provides a safe and effective method for promoting the growth and accumulation of arsenic in bulk crops sea cabbage and rapeseed by using new environmentally friendly nanoparticle SNPs, which is helpful for mitigating As poisoning and arsenic accumulation in plants. The effect of improving As accumulation is obvious

Method used

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  • Novel material and method for promoting plant growth and arsenic-absorbing accumulation
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  • Novel material and method for promoting plant growth and arsenic-absorbing accumulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A method for promoting growth and arsenic absorption and accumulation of sea kale, specifically comprising the following steps:

[0057] (1) Preparation of SNPs suspension

[0058] Accurately weigh 84 mg of SNPs with a sterilized weighing spoon and weighing paper, and pour into pre-sterilized 70ml DI H 2 In the Erlenmeyer flask of 0, prepare the SNPs mother liquor, after sealing the mouth, ultrasonically disperse for 60min, and place it on the ultra-clean workbench for subsequent use;

[0059] (2) Preparation of As(Ⅴ) solution

[0060] Accurately weigh Na with a sterilized weighing spoon and weighing paper 2 HASO 4 ·7H 2 O 0.0673g, pour into pre-sterilized 1mL DI H 2 Mix in a centrifuge tube of O, and place it in an ultra-clean workbench for subsequent use;

[0061] (3) Preparation of 1 / 2MS medium containing As and SNPs

[0062] After sterilizing 1 / 2MS medium, place it in an ultra-clean workbench to cool down, take 10ml of SNPs suspension and DI H 2 0. 30ml mixi...

Embodiment 2

[0068] A method for promoting growth and arsenic absorption and accumulation of sea kale, specifically comprising the following steps:

[0069] (1) Preparation of SNPs suspension

[0070] Accurately weigh 84 mg of SNPs with a sterilized weighing spoon and weighing paper, and pour into pre-sterilized 70ml DI H 2 In the Erlenmeyer flask of 0, prepare the SNPs mother liquor, after sealing the mouth, ultrasonically disperse for 60min, and place it on the ultra-clean workbench for subsequent use;

[0071] (2) Preparation of As(Ⅴ) solution

[0072] Accurately weigh Na with a sterilized weighing spoon and weighing paper 2 HASO 4 ·7H 2 O 0.0673g, pour it into a pre-sterilized centrifuge tube containing 1mL DI H2O and mix well, and place it in a clean bench for use;

[0073] (3) Preparation of 1 / 2MS medium containing As and SNPs

[0074] After sterilizing 1 / 2MS medium, place it in an ultra-clean workbench to cool down, take 10ml of SNPs suspension and DI H 2 0. 30ml mixing is mi...

Embodiment 3

[0080] A method for promoting growth and arsenic absorption and accumulation of sea kale, specifically comprising the following steps:

[0081] (1) Preparation of SNPs suspension

[0082] Accurately weigh 84 mg of SNPs with a sterilized weighing spoon and weighing paper, and pour into pre-sterilized 70ml DI H 2 In the Erlenmeyer flask of 0, prepare the SNPs mother liquor, after sealing the mouth, ultrasonically disperse for 60min, and place it on the ultra-clean workbench for subsequent use;

[0083] (2) Preparation of As(Ⅴ) solution

[0084] Accurately weigh Na with a sterilized weighing spoon 2 HASO 4 ·7H 2 O 0.0673g, pour it into a pre-sterilized centrifuge tube containing 1mL DIH2O and mix evenly, and place it in an ultra-clean workbench for later use;

[0085] (3) Preparation of 1 / 2MS medium containing As and SNPs

[0086] After sterilizing 1 / 2MS medium, place it in an ultra-clean workbench to cool down, take 10ml of SNPs suspension and DI H 2 0. 30ml mixing is mix...

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Abstract

The invention discloses a novel material and method for promoting plant growth and arsenic-absorbing accumulation, belongs to the technical field of environmental protection, and relates to a novel method for promoting plant growth and arsenic-absorbing accumulation by using sulfur nanoparticles. The nanoparticles have an excellent repairing effect on heavy metal-polluted water and heavy metal-polluted soil due to a large specific surface area, high catalytic performance, high adsorption performance and other capabilities of the nanoparticles. A new function of the novel environment-friendly nanomaterial sulfur nanoparticles (SNPs) is introduced, namely, the SNPs under the stress of different forms of As have great increase of arsenic accumulation in Crambe abyssinica and rape and promotion of growth of Crambe abyssinica and rape, the provided method is safe and effective, and can be used for promoting the growth and As accumulation of bulk crops of Crambe abyssinica and rape by usingthe novel environment-friendly nanoparticles SNPs, and theoretical support is provided for further researches and utilization of the sulfur nanoparticles in the field of restoration of As-polluted environments.

Description

technical field [0001] The invention belongs to the technical field of environmental protection and relates to a novel material and method for promoting plant growth and arsenic absorption and accumulation. More specifically, it relates to a novel approach to promote plant growth and arsenic uptake and accumulation using sulfur nanoparticles. Background technique [0002] Although my country has introduced a series of environmental protection and control measures in recent years, soil arsenic (As) still poses a serious threat to agricultural production, ecological environment and human health. Soil restoration work still needs to be strengthened urgently. However, in view of the actual national conditions of our country, it is extremely unrealistic to stop the farming of large areas of light and moderately heavy metal-polluted farmland, and to carry out long-term phytoremediation or other expensive physical and chemical engineering repairs. Therefore, for mildly and moderat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/02A01H4/00
CPCC09K17/02A01H4/008A01H4/001
Inventor 原海燕刘清泉张永侠王银杰孙玉明佟海英黄苏珍
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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