Cosmetic skincare compositions
A cosmetic composition and compound technology, applied in the field of cosmetic treatment, can solve the problems of skin barrier damage, low stratum corneum quality, damage and the like
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[0150] Interleukin-6 (IL-6) test
[0151] The biological activity of interleukin-6 (IL-6) in skin cells was measured. IL-6 is a cytokine and is used as a biomarker of inflammation. Skin cells (keratinocytes or fibroblasts) were subjected to UV stress to induce an IL-6 response in the presence of an active substance or a mixture of active substances to determine that the active substance or mixture of active substances inhibited IL-6. 6 Responsive activity.
[0152] The cultured skin cells (keratinocytes or fibroblasts) were seeded in a 96-well plate at a density of 5000 cells per well in cell growth medium with supplements, and incubated at 37°C for 24 hours. Determination of cell numbers for plating purposes is performed according to standard methods known in the art.
[0153] After incubation, the cell growth medium was replaced with 100 μl of PBS without calcium and magnesium and containing the desired relevant concentration of active substance(s). Controls included u...
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