Cadmium ion artificial antigen and application thereof
An artificial antigen and cadmium ion technology, applied in the field of immunochemical detection, can solve the problems of long analysis time, difficulty in adapting, and high cost of analysis methods, and achieve the effect of strong specificity, good sensitivity, and strong specificity
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Embodiment 1
[0044] (1) Synthesis of cadmium ion artificial antigen:
[0045] Weigh 6.5mg L 1 , dissolved in 2mL 0.01mol L -1 , pH 7.4 4-hydroxyethylpiperazineethanesulfonic acid HEPES solution, prepared L 1 Chelating agent solution, this solution is A liquid;
[0046] Weigh 88.66mg of cadmium nitrate and dissolve it in 5mL of ultrapure water to obtain a concentration of 7.5×10 -2 mol L -1 Cadmium nitrate solution, this solution is B liquid;
[0047] Add 175 μL of liquid B to liquid A dropwise, and react in the dark for 3 to 5 hours at room temperature, and this reaction liquid is liquid C;
[0048] Add 680 μL, 20 mmol L dropwise to solution C -1 glutaraldehyde aqueous solution, and react overnight at room temperature in the dark, and this reaction solution is D solution;
[0049] Weigh 20mg of bovine serum albumin BSA and dissolve it in 3mL of HEPES, stir magnetically at room temperature and mix well, this reaction solution is E solution;
[0050] Add solution D to solution E drop...
Embodiment 2
[0060] (1) Synthesis of cadmium ion artificial antigen:
[0061] Weigh 6.5mg L 1 , dissolved in 2mL 0.01mol L -1 , pH 7.4 4-hydroxyethylpiperazineethanesulfonic acid HEPES solution, prepared L 1 Chelating agent solution, this solution is A liquid;
[0062] Weigh 88.66mg of cadmium nitrate and dissolve it in 5mL of ultrapure water to obtain a concentration of 7.5×10 -2 mol L -1 Cadmium nitrate solution, this solution is B liquid;
[0063] Add 175 μL of liquid B to liquid A dropwise, and react in the dark for 3 to 5 hours at room temperature, and this reaction liquid is liquid C;
[0064] Add 680 μL, 20 mmol L dropwise to solution C -1 glutaraldehyde solution, react overnight at room temperature in the dark, this reaction solution is D solution;
[0065] Weigh 20mg chicken ovalbumin OVA and dissolve it in 3mL HEPES, stir magnetically at room temperature and mix well, this reaction solution is E solution;
[0066] Add solution D to solution E dropwise, react in the dark f...
Embodiment 3
[0076] The heterocyclic bifunctional chelating agent selected in this embodiment is L 2 , replacing L in Example 1 1 ; According to the same steps as in Example 1, the cadmium ion artificial antigen Cd-L was prepared 2 -BSA, cadmium ion artificial antigen synthesis route such as figure 2 As shown, the specific synthesis steps are as follows.
[0077] (1) Synthesis of cadmium ion artificial antigen:
[0078] Weigh 6.5mg L 2 , dissolved in 2mL 0.01mol L -1 , pH 7.4 4-hydroxyethylpiperazineethanesulfonic acid HEPES solution, prepared L 2 Chelating agent solution, this solution is A liquid;
[0079] Weigh 88.66mg of cadmium nitrate and dissolve it in 5mL of ultrapure water to obtain a concentration of 7.5×10 -2 mol L -1 Cadmium nitrate solution, this solution is B liquid;
[0080] Add 175 μL of liquid B to liquid A, and react in the dark for 3 to 5 hours at room temperature, and this reaction liquid is liquid C;
[0081] Add 680 μL, 20 mmol L dropwise to solution C -1 ...
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