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Method for studying action mechanism of Hsa_circ_0111659 in repairing of ESC by WJ-MSCs

A mechanism of action, wj-mscs technology, applied in medical preparations containing active ingredients, biochemical equipment and methods, applications, etc., can solve problems that have not been reported in the literature

Pending Publication Date: 2019-11-05
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether circRNAs are also involved in the process of endometrial injury repair and play a role in transcriptional regulation has not been reported in the literature so far.

Method used

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  • Method for studying action mechanism of Hsa_circ_0111659 in repairing of ESC by WJ-MSCs
  • Method for studying action mechanism of Hsa_circ_0111659 in repairing of ESC by WJ-MSCs
  • Method for studying action mechanism of Hsa_circ_0111659 in repairing of ESC by WJ-MSCs

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Embodiment Construction

[0179] An application of Hsa_circ_0111659 in the preparation of WJ-MSCs to repair damaged endometrium.

[0180] Materials and Methods

[0181] 2.1 Research materials and equipment

[0182] 2.1.1 Source of cell tissue

[0183] (1) Umbilical cord tissue: derived from normal full-term cesarean section newborns. (Provided by the Affiliated Hospital of Nantong University)

[0184] (2) Endometrium: from patients suffering from uterine fibroids (except submucosal fibroids) with regular menstrual cycles before surgery, no intrauterine device placed, no hormone therapy six months before surgery, and no combination of other diseases Internal and surgical diseases and postoperative pathology confirmed no endometrial lesions. (Provided by the Affiliated Hospital of Nantong University)

[0185] 2.1.2 Main instruments

[0186] (1) Vertical pressure steam sterilizer (Japan SANYO company)

[0187] (2) Ultra-low temperature freezer (Japan SANYO company)

[0188] (3) CO2 cell incubator ...

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Abstract

The invention discloses a method for studying an action mechanism of Hsa_circ_0111659 in repairing of ESC by WJ-MSCs. According to the method, it is found that the hsa_circ_0111659 plays a positive regulatory role in repairing of injured stromal cells by the WJ-MSCs, and the proliferation of ESC can be reduced and the number of apoptotic cells in ESC can be increased by knocking down the hsa_circ_0111659. A targeting axis, namely the hsa_circ_0111659 / miR-20b-5p / VEGF plays an important role in repair of endometria, and the hsa_circ_0111659 provides a new idea for the repair of endometrial stromal cells.

Description

technical field [0001] The present invention relates to a method for studying the application of Hsa_circ_0111659 and its mechanism of action. Background technique [0002] The integrity of endometrial function is of great significance to female reproductive function. Endometrial repair disorder caused by mechanical injury or infection in the uterine cavity has become an important cause of intrauterine adhesions, amenorrhea, and infertility, seriously affecting women's menstrual cycle and normal pregnancy. Current studies suggest that the maintenance of a normal menstrual cycle mainly depends on the basal layer of the endometrium. The damage to the basal layer of the endometrium can change the regular growth and shedding of the endometrium in the normal menstrual cycle, and the damage to the basal layer leads to difficulties in the formation of new blood vessels, which makes it difficult for the endometrium to achieve self-repair. Since basal layer cells cannot regenerate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12N5/071C12N5/0775A61K31/7105A61P15/00
CPCG01N33/5008G01N33/5044C12N5/0682C12N5/0668A61K31/7105A61P15/00C12N2502/00C12N2503/00
Inventor 张玉泉杨晓清孙宝兰施沁施蕾叶青
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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