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Freeze drying method for improving survival rate of lactobacillus plantarum

A technology of Lactobacillus plantarum and drying method, which is applied in the field of freeze-drying to improve the survival rate of Lactobacillus plantarum, and can solve problems such as abnormal regulation of physiological metabolism, cell dehydration, and death

Pending Publication Date: 2019-11-08
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the freeze-drying process, when the temperature drops and the vacuum increases, the water in the cells freezes and evaporates, and the solutes and electrolytes in the cells are gradually concentrated, resulting in excessive water loss of cells, severe shrinkage and deformation, and even death; in addition, When the electrolyte is highly concentrated, the high-level structure of some electrolyte-sensitive proteins in the cell will change, especially the key enzymes related to metabolism, which will lose their physiological functions, causing abnormal regulation of physiological metabolism, and resulting in cell freeze-drying decline in survival

Method used

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  • Freeze drying method for improving survival rate of lactobacillus plantarum
  • Freeze drying method for improving survival rate of lactobacillus plantarum

Examples

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Embodiment 1

[0032] figure 1 It is a flow chart of freeze-drying in the embodiment of the freeze-drying method that the present invention improves the plant lactobacillus survival rate.

[0033] Such as figure 1 Shown, a kind of freeze-drying method that improves plantaractobacillus survival rate, the steps are as follows:

[0034] In step 1, the Lactobacillus plantarum AR113 was repeatedly streaked and activated 3 times in the solid MRS medium to obtain a single colony of the Lactobacillus plantarum AR113;

[0035] Step 2, pick the activated single colony and inoculate it for 12 hours to obtain the seed solution;

[0036] Step 3, the seed solution is transferred to the MRS liquid medium containing 1g / L oleic acid for expansion and cultivation, and at 37°C for expansion and cultivation for 12h, the expansion culture solution is obtained;

[0037] Step 4, use a microplate reader to adjust the concentration of the expanded culture solution to make it OD 600 =1, under the condition that t...

Embodiment 2

[0040] figure 1 It is a flow chart of freeze-drying in the embodiment of the freeze-drying method that the present invention improves the plant lactobacillus survival rate.

[0041] Such as figure 1 Shown, a kind of freeze-drying method that improves plantaractobacillus survival rate, the steps are as follows:

[0042] In step 1, the Lactobacillus plantarum AR307 was repeatedly streaked and activated 3 times in the solid MRS medium to obtain a single colony of the Lactobacillus plantarum AR307;

[0043] Step 2, pick the activated single colony and inoculate it for 12 hours to obtain the seed solution;

[0044] Step 3, the seed solution is transferred to the MRS liquid medium containing 1g / L oleic acid for expansion and cultivation, and at 37°C for expansion and cultivation for 12h, the expansion culture solution is obtained;

[0045] Step 4, use a microplate reader to adjust the concentration of the expanded culture solution to make it OD 600 =1, under the condition that t...

Embodiment 3

[0048] figure 1 It is a flow chart of freeze-drying in the embodiment of the freeze-drying method that the present invention improves the plant lactobacillus survival rate.

[0049] Such as figure 1 Shown, a kind of freeze-drying method that improves plantaractobacillus survival rate, the steps are as follows:

[0050] In step 1, Lactobacillus plantarum WCFS1 was activated by streaking repeatedly in solid MRS medium for 3 times to obtain a single colony of Lactobacillus plantarum WCFS1;

[0051] Step 2, pick the activated single colony and inoculate it for 12 hours to obtain the seed solution;

[0052] Step 3, the seed solution is transferred to the MRS liquid medium containing 1g / L oleic acid for expansion and cultivation, and at 37°C for expansion and cultivation for 12h, the expansion culture solution is obtained;

[0053] Step 4, use a microplate reader to adjust the concentration of the expanded culture solution to make it OD 600 =1, under the condition that the centr...

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Abstract

The invention provides a freeze drying method for improving the survival rate of lactobacillus plantarum and belongs to the field of freeze drying. The method provided by the invention comprises the following steps: step 1, performing strain activating to obtain a single colony; step 2, inoculating and culturing the single colony to obtain a seed liquid; step 3, transferring the seed liquid to anMRS liquid culture medium containing 0.5-2 g / L octadecenoic acid for expanding culture to obtain an expanded culture solution; step 4, centrifuging the expanded culture solution to obtain thallus precipitate; and step 5, washing the thallus precipitate with a PBS buffer solution, re-suspending the thallus precipitate in a PBS solution or a protective agent solution, transferring the thallus precipitate into a container, and freeze-drying the thallus precipitate. The octadecenoic acid is preferably oleic acid. The octadecenoic acid is used in the expanding culture step, so that the survival rate of the rehydrated lactobacillus plantarum after freeze drying is obviously improved, and unexpected technical effects are brought.

Description

technical field [0001] The invention relates to a freeze-drying method, in particular to a freeze-drying method for improving the survival rate of Lactobacillus plantarum, and belongs to the field of freeze-drying. Background technique [0002] Water is indispensable for the metabolism of the body and the maintenance of life processes. In the absence of water, living cells are dead or dormant. Therefore, in order to stabilize unstable products such as probiotics, the water content in stored samples must be reduced to a dormant state. Freezing is a unit operation that freezes moisture, thereby reducing the water content of the sample to a dormant state. However, maintaining and shipping samples in a frozen state is expensive and may result in loss of value of the product. Alternatively, samples can be dried in air at high processing temperatures, but traditional drying methods can cause changes in the physical and chemical properties of the sample. Freeze-drying just comb...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/25
CPCC12N1/20C12N1/04
Inventor 王光强艾连中夏永军熊智强张汇
Owner UNIV OF SHANGHAI FOR SCI & TECH
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