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Transglutaminase fermentation culture medium pretreated by acidolysis or enzymolysis and preparation method and application thereof.

A technology of transglutaminase and fermentation medium is applied in the fields of microbial fermentation and enzyme engineering, and can solve the problems of low utilization rate of fish meal, low efficiency of fermentation and enzyme production, etc.

Inactive Publication Date: 2019-11-15
TAIXING DONGSHENG FOOD TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention overcomes the problems of low utilization rate of fish meal and low efficiency of fermenting enzyme production in the transglutaminase fermentation medium in the prior art, etc.

Method used

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  • Transglutaminase fermentation culture medium pretreated by acidolysis or enzymolysis and preparation method and application thereof.
  • Transglutaminase fermentation culture medium pretreated by acidolysis or enzymolysis and preparation method and application thereof.
  • Transglutaminase fermentation culture medium pretreated by acidolysis or enzymolysis and preparation method and application thereof.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] The preparation method of embodiment 1 transglutaminase fermentation medium

[0087] Follow the steps below.

[0088] 1) Ingredients: Weigh the fish meal and water and mix them evenly to obtain the mixing system 1;

[0089] 2) Adjust the pH: Stir the mixed system 1 with a magnetic stirrer, and adjust the corresponding pH to the range of 1.5-8.0pH with the corresponding acid to obtain the mixed system 2;

[0090] 3) Enzyme addition: adding hydrolytic enzymes according to the weight ratio to obtain the mixed system 3;

[0091] 4) Hydrolysis: After placing the mixed system 3 at the corresponding temperature of 35°C-50°C for a certain period of time, immediately inactivate the enzyme at ultra-high temperature, and obtain the transglutaminase fermentation medium to be used after cooling. Fishmeal ingredients pretreated by enzymatic hydrolysis, that is, the mixed system four.

[0092] 5) Preparation: transfer the mixed system IV to a container, and stir the mixed system IV...

Embodiment 2

[0106] The preparation of embodiment 2 transglutaminase fermentation medium

[0107] Follow the steps below.

[0108] 1) Ingredients: Weigh the fish meal and water and mix them evenly to obtain the mixing system 1;

[0109] 2) Adjust the pH: Stir the mixed system 1 with a magnetic stirrer, and adjust the corresponding pH to the range of 1.5-8.0pH with the corresponding acid to obtain the mixed system 2;

[0110] 3) Enzyme addition: adding hydrolytic enzymes according to the weight ratio to obtain the mixed system 3;

[0111] 4) Hydrolysis: after placing the mixed system 3 at the corresponding action temperature of 35°C-50°C for a certain period of time, the enzyme will be instantly inactivated by ultra-high temperature, and after cooling, obtain the transglutaminase fermentation medium to be used Fishmeal ingredients pretreated by enzymatic hydrolysis, that is, the mixed system four.

[0112] 5) Preparation: transfer the mixed system IV to a container, and stir the mixed sy...

Embodiment 3

[0126] The preparation of embodiment 3 transglutaminase fermentation medium

[0127] Follow the steps below.

[0128] 1) Ingredients: Weigh the fish meal and water and mix them evenly to obtain the mixing system 1;

[0129] 2) Adjust the pH: Stir the mixed system 1 with a magnetic stirrer, and adjust the corresponding 1.5-8.0pH to the action pH range with the corresponding acid to obtain the mixed system 2;

[0130] 3) Enzyme addition: adding hydrolytic enzymes according to the weight ratio to obtain the mixed system 3;

[0131] 4) Hydrolysis: after placing the mixed system 3 at the corresponding temperature of 35°C-50°C for a certain period of time, immediately inactivate the enzyme at ultra-high temperature, and obtain the transglutaminase fermentation medium to be used after cooling. The enzymatically hydrolyzed fishmeal component, that is, the mixed system four.

[0132] 5) Preparation: transfer the mixed system IV to a container, and stir the mixed system IV with a mag...

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Abstract

The invention discloses a transglutaminase fermentation culture medium pretreated by acidolysis or enzymolysis and a preparation method and application thereof. According to the method, fish meal components in the culture medium are separately subjected to acid hydrolysis and enzymatic hydrolysis, correspondingly nutrient components in the fish meal components can be utilized by a transglutaminaseproducing strain, the utilization rate of the nutrient components by the transglutaminase producing strain is increased, and the enzyme production efficiency is thus improved. The method has the advantages of simple and convenient operation, high efficiency, low cost and easy application in industrial production.

Description

technical field [0001] The invention relates to the field of microbial fermentation and enzyme engineering, and relates to a transglutaminase fermentation medium pretreated by acid hydrolysis or enzymolysis, a preparation method and application thereof. Background technique [0002] Transglutaminase, a transferase that catalyzes the acyl transfer reaction, can promote protein intramolecular cross-linking, protein intermolecular cross-linking and cross-linking between proteins and amino acids, which can greatly improve protein The functional properties of protein can improve the nutritional value of protein, and it has a wide range of applications in the food processing industry. Therefore, the production of transglutaminase has huge market demand and application development value. [0003] Transglutaminase was first discovered in the liver of guinea pigs. It was first isolated from Streptomyces maoyuan in 1989. It was produced on a large scale in 1997. It was officially lis...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N1/20C12R1/465
CPCC12N9/1044C12Y203/02013C12N1/20
Inventor 步国建张玉如甘李寇雪蕊和超楠刘艳贺晓洁
Owner TAIXING DONGSHENG FOOD TECH
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