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Construction method and application of mouse model of osteosarcoma xenograft

A mouse model and xenotransplantation technology, which is applied in the field of establishment of tumor transplantation mouse model, achieves the effect of real situation, reduction of treatment cost and government financial expenditure, and easy tumor metastasis

Pending Publication Date: 2019-11-22
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0007] This invention solves the problem of tumor growth efficiency in xenograft mouse models, but there is still a need in the art for more types of osteosarcoma xenograft mouse models and their construction methods

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  • Construction method and application of mouse model of osteosarcoma xenograft
  • Construction method and application of mouse model of osteosarcoma xenograft
  • Construction method and application of mouse model of osteosarcoma xenograft

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Embodiment Construction

[0031] After review and approval by the hospital ethics committee, all patients signed an informed consent before sample collection. The collected tissues undergo histopathological examination. The tumor tissues of patients with osteosarcoma confirmed pathologically are stored in sterile saline and placed on ice . The tissue block is divided into 3 parts. The first part is directly processed under aseptic conditions (see below) for xenotransplantation into immunodeficient mice; the second part is implanted with 10% DMSO (dimethyl sulfoxide), 89% Among FBS (fetal bovine serum) and 1% NEAA (non-essential amino acid) DMEM, DMEM medium is a medium containing various amino acids and glucose, which is gradient frozen to -80°C and stored in liquid nitrogen; The 3 parts are quickly frozen in liquid nitrogen and then stored in liquid nitrogen. Among them, the first part is used for modeling, the second part is used for resuscitation and vaccination, and the third part is used for genet...

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Abstract

The invention provides a construction method and application of a mouse model of osteosarcoma xenograft. The method comprises the steps that firstly, a tumor tissue block of an osteosarcoma patient iscollected, and then the fresh tumor tissue block of the patient is subjected to orthotopic xenograft into the immunodeficient mouse body under the aseptic condition, wherein the orthotopic xenograftcomprises the steps that a 2-6-week-old immunodeficient mouse is selected, the sex of the mouse keeps consistent with the sex of the patient, the collected fresh tumor tissue block of the patient is soaked in a sterile incubation solution, the mouse is fixed after anesthesia, the tumor tissue block of the patient is implanted into the marrow cavity of the mouse, an operative incision on the skin of the mouse is sutured, and the mouse is raised, so that the tumor in the marrow cavity of the mouse grow. Compared with subcutaneous modeling, orthotopic modeling is realer, the screened drug schemecan be used in the patient better, and orthotopic modeling is more likely to cause tumor metastasis than subcutaneous modeling in theory.

Description

Technical field [0001] The invention relates to the field of establishment of tumor transplantation mouse models, in particular to a method for constructing an osteosarcoma xenotransplantation mouse model and its application. Background technique [0002] Osteosarcoma is the most common primary bone malignant tumor in Hunan Province. It is extremely malignant, prone to lung metastasis, extremely high disability and mortality, and extremely poor prognosis. Seventy-five percent of osteosarcoma patients are adolescents. Once discovered, amputations are often used. Even so, the 5-year survival rate after amputation is only 5-20%. This brings great mental harm to patients and their families, and also brings heavy economic burdens to society and the country, and brings greater social problems. Because of the heterogeneity of osteosarcoma tissues, only less than 20% of the results of previous basic research based on osteosarcoma cell lines can be reproduced in clinical osteosarcoma pat...

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Application Information

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IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/0271A61K49/0008A01K2207/12A01K2207/15A01K2227/105A01K2267/0331A01K2267/0393
Inventor 黎志宏涂超任晓磊陈瑞奇刘忠越张程豪
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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