A kind of preparation method and application of modified melaleuca polysaccharide
A technology of Melaleuca tower and tower polysaccharide, which is applied in the field of preparation of Melaleuca tower polysaccharide, can solve problems such as poor water solubility of Melaleuca tower polysaccharide, and achieve the effects of high degree of substitution, improved anti-oxidation, good efficacy and market competitiveness.
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[0039] The preparation method of a modified melaleuca polysaccharide proposed by the invention can prepare a modified melaleuca polysaccharide. see figure 1 , the preparation method of the modified melaleuca polysaccharide comprises the following steps:
[0040] S10, dissolving the melaleuca polysaccharide in a sodium hydroxide solution to obtain a melaleuca polysaccharide solution;
[0041] S20, adding a modifier to the melaleuca polysaccharide solution, stirring and reacting to obtain a reaction solution;
[0042] S30. After adjusting the reaction solution to neutrality, dialyze to obtain a concentrated solution;
[0043] S40, after alcohol precipitation treatment of the concentrated solution, solid-liquid separation is performed, the precipitate is collected and dried to obtain modified melaleuca polysaccharide;
[0044] Wherein, the modifying agent is a phosphorylation reagent or a carboxymethylation reagent.
[0045] In this embodiment, in step S10, an alkaline soluti...
Embodiment 1
[0065] Crush Melaleuca polysaccharide into Melaleuca polysaccharide powder, take 0.1g Melaleuca polysaccharide powder, add sodium hydroxide solution (containing 0.8g sodium hydroxide), alkalinize for 1 hour until Melaleuca polysaccharide is fully dissolved, and obtain Melaleuca Polysaccharide solution. Under stirring at 100°C, add 0.4g phosphorylation reagent (the mass ratio of sodium tripolyphosphate to sodium trimetaphosphate is 7:1) to the melaleuca polysaccharide solution, adjust the reaction solution to pH 7, and react 7.0 h to get the reaction solution. The reaction solution was placed in a dialysis bag with a molecular weight cut-off of 3500 for dialysis. When no white precipitate was produced after silver nitrate solution was added to the dialysis solution, the dialysis was stopped and the concentrated solution was collected. Add 4 times the volume of concentrated solution of ethanol to the concentrated solution, let stand overnight, and then centrifuge. The precipit...
Embodiment 2
[0067] Crush Melaleuca polysaccharide into Melaleuca polysaccharide powder, take 0.1g Melaleuca polysaccharide powder, add sodium hydroxide solution (containing 1g of sodium hydroxide), alkalinize for 0.7h until Melaleuca polysaccharide is fully dissolved, and obtain Melaleuca Polysaccharide solution. Add 1g of phosphorylation reagent (the mass ratio of sodium tripolyphosphate to sodium trimetaphosphate is 6:1) to the melaleuca polysaccharide solution under stirring at 70°C, adjust the reaction solution to pH 7, and react for 5.0h get the reaction solution. The reaction solution was placed in a dialysis bag with a molecular weight cut-off of 3500 for dialysis. When no white precipitate was produced after silver nitrate solution was added to the dialysis solution, the dialysis was stopped and the concentrated solution was collected. Add 3 times the volume of concentrated liquid ethanol to the concentrated liquid, let stand overnight, and then centrifuge. The precipitate was v...
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