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Application of natural small molecular compound to inducing osteogenic differentiation of umbilical cord mesenchymal stem cells

A technology of small molecule compounds and mesenchymal stem cells, applied in the field of stem cells, can solve problems such as no report on the activity of mesenchymal stem cells

Pending Publication Date: 2019-11-22
上海容音医疗科技咨询中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Polygalic acid is a natural small molecular compound in Polygala, and there is no report on its activity in the field of mesenchymal stem cells

Method used

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  • Application of natural small molecular compound to inducing osteogenic differentiation of umbilical cord mesenchymal stem cells
  • Application of natural small molecular compound to inducing osteogenic differentiation of umbilical cord mesenchymal stem cells
  • Application of natural small molecular compound to inducing osteogenic differentiation of umbilical cord mesenchymal stem cells

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Embodiment Construction

[0018] The substantive content of the present invention is set forth below through specific examples, but the protection scope of the present invention cannot be limited thereto.

[0019] 1. Experimental materials

[0020] Fetal bovine serum (FBS) and α-MEM medium were purchased from Gibco, USA.

[0021] Type II collagenase was purchased from Beijing Biolab Technology Co., Ltd.

[0022] Trypsin from Shanghai Shifeng Biotechnology Co., Ltd.

[0023] Green chain double antibody Shanghai Xiaopeng Biotechnology Co., Ltd.

[0024] Polygalic acid was purchased from Chengdu Ruifensi Biotechnology Co., Ltd., CAS#: 22338-71-2, HPLC purity ≥ 98%.

[0025] 2. Experimental method

[0026] 1. Isolation and identification of umbilical cord mesenchymal stem cells

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Abstract

The invention discloses application of natural small molecular compound to inducing osteogenic differentiation of umbilical cord mesenchymal stem cells. According to the application, the natural smallmolecular compound polygalic acid can not only promote proliferation of the umbilical cord mesenchymal stem cells in vitro, but also promote osteogenic differentiation of the umbilical cord mesenchymal stem cells; that is to say, after the umbilical cord mesenchymal stem cells are subjected to in-vitro culture in a culture medium containing polygalic acid, bone tissue engineering cells with a quantitative advantage can be obtained, and used for bone tissue engineering and regenerative medicine.

Description

technical field [0001] The invention belongs to the field of stem cells and relates to the proliferation and induced differentiation of mesenchymal stem cells, in particular to the use of a natural small molecule compound to induce osteogenic differentiation of umbilical cord mesenchymal stem cells. Background technique [0002] The incidence of bone defects has been high, and the resulting limb dysfunction will seriously affect the quality of life of patients. Although bone transplantation can be used as a treatment, there are problems such as shortage of supply sources, low complete healing rate, postoperative complications and immune rejection, which limit its clinical application. Tissue engineering and regenerative medicine centered on stem cells have brought new hope. [0003] Mesenchymal stem cells (MSCs) are pluripotent stem cells with self-renewal ability. They have the characteristics of immune regulation function, abundant sources, and easy acquisition. They are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/0654C12N2500/30C12N2506/1369
Inventor 蔡凤梅陈亮
Owner 上海容音医疗科技咨询中心
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