Absorbable bone wax and preparation method thereof
A bone wax and bone repair technology, applied in the field of biomedical engineering materials, can solve the problems of destroying surrounding tissue, unable to repair bone tissue, affecting bone regrowth, etc., achieving strong antibacterial and bacteriostatic effects, reducing inflammation, and inducing bone The effect of hyperplasia
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[0044] In some embodiments, the present invention provides a method for preparing hemostatic starch microspheres, comprising the steps of:
[0045] Weigh a certain amount of soluble starch, add an appropriate amount of purified water, mix thoroughly, adjust the pH with anhydrous sodium carbonate, heat and stir on a magnetic stirrer, gelatinize until transparent, and cool for later use. Weigh a certain amount of cross-linking agent and put it in another beaker, add an appropriate amount of purified water, stir and dissolve on a magnetic stirrer, pour it into the gelatinized starch solution after dissolution, stir evenly, and set aside. Add an appropriate amount of soybean oil into the three-necked flask and then add the emulsifier Span80, put it into a constant temperature water bath and heat it to 60°C to completely dissolve the Span80, then cool it down to 45°C and add the prepared starch solution slowly under mechanical stirring. When the starch solution is added dropwise, r...
Embodiment 1
[0084] The preparation of embodiment 1 hemostatic starch microspheres
[0085] Weigh 24g of soluble starch, add an appropriate amount of purified water and mix thoroughly to prepare 8wt% starch solution, adjust pH≧10 with anhydrous sodium carbonate, heat to 60°C on a magnetic stirrer, and stir at a speed of 400r / min , gelatinized until transparent, cooled for later use. Weigh 2.4g of the cross-linking agent and put it in another beaker, add an appropriate amount of purified water, stir and dissolve on a magnetic stirrer, pour it into the gelatinized starch solution after dissolution, stir evenly, and set aside. According to the water / oil ratio of 1 / 2, add 600ml of soybean oil into the three-necked flask and then add 5.4g of emulsifier Span80, put it in a constant temperature water bath and heat it to 60°C to completely dissolve Span80, then cool it down to 45°C under mechanical stirring , Slowly add the prepared starch solution. When the starch solution is added dropwise, re...
Embodiment 2
[0087] Preparation of Example 2 β-tricalcium phosphate (β-TCP) bone repair material
[0088] Weigh a certain amount of Ca(NO 3 ) 2 , and dissolve it in a beaker containing deionized water to prepare a 0.5mol / L solution; then weigh a certain amount of (NH 4 ) 2 HPO 4 and dissolve it in a beaker containing deionized water to prepare a 0.5mol / L solution; add an appropriate amount of SDS to Ca(NO 3 ) 2 In the solution, under 400~600rpm mechanical stirring, according to Ca(NO 3 ) 2 with (NH 4 ) 2 HPO 4 The molar ratio of 3:1 was added dropwise (NH 4 ) 2 HPO 4 solution; in dropwise addition (NH 4 ) 2 HPO 4 In the process of solution, add a certain amount of ammoniacal liquor, make whole system keep pH ≥ 10 in reaction process; 4 ) 2 HPO 4 After the solution was added dropwise, continue to react for 2 hours under mechanical stirring, and then let the precipitation stand for one night; then use a Buchner funnel to carry out suction filtration, and then alternately wa...
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