A chemiluminescent signal time and space two-dimensional resolution detection device and detection method
A chemiluminescence, detection device technology, applied in chemiluminescence/bioluminescence, measurement device, analysis by chemical reaction of materials, etc., can solve the problem of insufficient development and utilization of microfluidic paper chip spatial resolution capability, difficult to adapt to The development of portable instant detection devices and the high price of instruments have achieved the effects of wide popularization and promotion, short detection time and low detection cost.
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Embodiment 1
[0092] In this preparation example, a figure 1 The two-dimensional monolayer chemiluminescence time- and space-resolved detection paper chip shown, figure 1 Blue areas represent hydrophilic flow channels and gray areas represent hydrophobic regions. The specific production process is as follows:
[0093] 1) First use CAD drawing software to design the hydrophilic flow channel pattern of the paper chip for detection (see attached figure 1 shown), including designing the shape, size and location of injection holes, flow channels and reaction wells.
[0094] The design parameters of the paper chip are as follows:
[0095] The injection hole is a circle with a diameter of 10 mm, and the first-stage annular flow channel is derived from the injection hole in an arc shape. The width of the first-stage annular circulation channel is 3 mm. Four secondary (second, third, fourth, and fifth) flow channels are branched from the circular flow channel at intervals of 10 mm, and each sec...
Embodiment 2
[0107] In this example, an attached Figure 5 The three-dimensional double-layer stereotype chemiluminescent time- and space-resolved detection paper chip shown, Figure 5Blue areas represent hydrophilic flow channels and gray areas represent hydrophobic regions. The specific production process is as follows:
[0108] 1) First use CAD drawing software to design the circulation channel pattern of each functional layer of the three-dimensional double-layer detection paper chip (see attached Figure 5 shown), including designing the shape, size, and location of injection holes, secondary injection holes, flow-through channels, and reaction wells.
[0109] 2) According to the attached Figure 5 Design the pattern, use the 200-mesh nylon mesh as the substrate, and the impermeable photosensitive emulsion as the coating to prepare the screen printing template. Using paraffin as a hydrophobic material, the designed wax patterns of the sampling functional layer and the reaction fun...
Embodiment 3
[0120] Embodiment 3 (comparative example):
[0121] In this example, a three-dimensional double-layer stereotype chemiluminescent time- and space-resolved detection paper chip (as attached Figure 8 shown). The steps of this embodiment are similar to those of Embodiment 2, the only difference being that the stacking sequence of the sampling function layer and the reaction function layer is different. The inventor used another self-made luminescence detection cassette, denoted as detection cassette 2, to perform chemiluminescence time and space two-dimensional resolution detection. The specific method is as follows:
[0122] 1) First, prepare the sampling functional layer and the reaction functional layer of the microfluidic paper chip according to the steps 1), 2) and 3) of Example 2.
[0123] 2) Prepare the two-layer paper chip with hydrophilic flow channel according to Figure 8 In the method shown, the first functional layer and the second functional layer are aligned a...
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