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Anisaldehyde/diarginine composite antibacterial agent, and preparation method and application thereof

A compound antibacterial agent and double arginine technology, which is applied in the field of anisaldehyde/double arginine compound antibacterial agent and its preparation, can solve the problems of less research on anisaldehyde antibacterial, achieve simple and economical preparation method, and good bactericidal effect Effect

Inactive Publication Date: 2019-12-13
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been few researches on the antibacterial effect of p-anisaldehyde at home and abroad.

Method used

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  • Anisaldehyde/diarginine composite antibacterial agent, and preparation method and application thereof
  • Anisaldehyde/diarginine composite antibacterial agent, and preparation method and application thereof
  • Anisaldehyde/diarginine composite antibacterial agent, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Evaluation of antibacterial performance of anisaldehyde / double arginine compound antibacterial agent against Escherichia coli O157 in lettuce

[0019] 1 Experimental materials

[0020]

[0021]

[0022] 2 Experimental methods

[0023] 1) Preparation of anisaldehyde / double arginine compound antibacterial agent

[0024] According to the preparation of 0.6% of anisaldehyde, 3% of ether, 1% of bis-arginine, and the balance is sterile distilled water, it is fully dissolved by ultrasonication for 30 minutes under 60W power to obtain a compound antibacterial agent.

[0025] 2) Antibacterial effect of compound antibacterial agent

[0026] (1) Escherichia coli O157 was inoculated in a nutrient liquid medium, and cultured at 37° C. with shaking (150 rpm) for 2 days to obtain Escherichia coli O157 in logarithmic growth phase.

[0027] (2) Three antibacterial agents (anisaldehyde / bis-arginine composite antibacterial agent, 0.6% anisaldehyde, 1% bis-arginine) were mixed wit...

Embodiment 2

[0041] Evaluation of antibacterial performance of anisaldehyde / bis-arginine compound antibacterial agent against Listeria monocytogenes in cheese

[0042] 1 Experimental materials

[0043]

[0044] 2 Experimental methods

[0045] 1) Preparation of anisaldehyde / double arginine compound antibacterial agent

[0046] According to the composition of 0.6% anisaldehyde, 3% diethyl ether, 1% bis-arginine, and the balance is sterile distilled water, it is fully dissolved by ultrasonication for 30 minutes under 60W power to obtain a compound antibacterial agent solution.

[0047] 2) antibacterial effect of compound antibacterial agent in PBS

[0048] (1) Listeria monocytogenes was inoculated in a nutrient liquid medium, and cultured with shaking (150 rpm) at 25° C. for 2 days to obtain Listeria monocytogenes in logarithmic growth phase.

[0049] (2) Three antibacterial agents (anisaldehyde / bis-arginine composite antibacterial agent, 0.6% anisaldehyde, 1% bis-arginine) were mixed w...

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Abstract

The invention belongs to the field of preparation of antibacterial agents, and particularly relates to an anisaldehyde / diarginine composite antibacterial agent, and a preparation method and an application thereof. Diarginine, anisaldehyde, ethanol and sterile distilled water are mixed according to a certain ratio to prepare the anisaldehyde / diarginine composite antibacterial agent, so that the preparation cost of the antibacterial agent can be reduced, and the antibacterial performance of the antibacterial agent can be improved. The novel composite antibacterial agent can effectively kill various food-borne pathogenic bacteria, such as escherichia coli O157 and listeria monocytogenes. Compared with single diarginine (anisaldehyde), the composite antibacterial agent has a good antibacterialeffect in application of vegetables and cheese.

Description

technical field [0001] The invention belongs to the field of preparation of food antibacterial agents, and in particular relates to an anisaldehyde / bisarginine composite antibacterial agent, its preparation method and application. Background technique [0002] Foodborne pathogens are pathogenic bacteria that can cause food poisoning. Common foodborne pathogens include Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli. In order to solve the harm brought to people by food-borne pathogens, the use of chemical additives has become the first choice of antibacterial drugs. In this context, there is an urgent need for a safe and non-toxic antibacterial agent with broad-spectrum antibacterial effect in the field of food antibacterial agent preparation. However, with the wide application of chemical additives and antibacterial agents, the problem of drug resistance has become increasingly prominent. Therefore, the research of natural antibacterial agents has beco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154A23C19/10A23L3/3499A23L3/3526
CPCA23B7/154A23C19/10A23L3/3499A23L3/3526A23V2002/00
Inventor 林琳廖雪崔海英柏梅
Owner JIANGSU UNIV
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