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Application of a kind of outer membrane protein in the preparation of immunotherapeutic drugs for malignant tumors

An immunotherapy drug and outer membrane protein technology, applied in the field of medicine, can solve problems such as unrevealed effects

Active Publication Date: 2021-08-20
君维安(武汉)生命科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The patent application number CN008050937 discloses the use of enterobacterial OmpA protein combined with antigen for generating anti-viral, anti-Jiazoan, or anti-tumor cytotoxic response, which corresponds Klebsiella pneumoniae membrane protein to tumor Antigens are combined to prepare combined drugs to treat tumors. The patent application number 2010105017674 discloses the application of SJ16 protein in the preparation of immunosuppressive drugs. It uses SJ16 protein to prepare immunosuppressive drugs. Through the immune tolerance mechanism and induction, it can be used to a large extent. To avoid toxic and side effects, although it can inhibit the transformation and proliferation of tumor cells, it does not reveal the impact on the microenvironmental effect T lymphocytes in tumor cell immunotherapy, which has great shortcomings in the microenvironment regulation of tumor immunotherapy

Method used

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  • Application of a kind of outer membrane protein in the preparation of immunotherapeutic drugs for malignant tumors
  • Application of a kind of outer membrane protein in the preparation of immunotherapeutic drugs for malignant tumors
  • Application of a kind of outer membrane protein in the preparation of immunotherapeutic drugs for malignant tumors

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Embodiment 1

[0043] Example 1: Materials and methods

[0044] 1. Outer membrane protein expression

[0045] 1. Use Escherichia coli engineering bacteria BL21 strain for heterologous expression, high-efficiency expression of genes cloned in expression vectors (such as pET series) containing phage T7 promoter, the plasmid PET 28a-Amuc used in this experiment, the map is as attached figure 1 , the Amuc protein gene is introduced into PET 28a through genetic engineering, and the plasmid PET 28a-Amuc containing the Amuc protein gene is constructed. The method for constructing the plasmid is conventional genetic engineering technology, and will not be repeated here. Cultivate overnight on LB+KanR resistant plates, select monoclonal antibodies, transfer to 100 ml LB+KanR resistant liquid medium, overnight, transfer to 200ml LB+KanR resistant liquid medium at a ratio of 1:100, Cultivate at 37°C for 2-3 hours, so that OD=0.6, 8000r / min, centrifuge for 5min, discard the supernatant, and store the b...

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Abstract

The invention discloses an application of an outer membrane protein in the preparation of immunotherapy drugs for malignant tumors. The outer membrane protein is an Akkermansia outer membrane protein. The outer membrane protein provided by the application can reduce tumor burden and inhibit tumor growth. The activation of T cell-related anti-tumor immune response can be applied to the preparation of various malignant tumor immunotherapy drugs.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of an outer membrane protein in the preparation of immunotherapeutic drugs for malignant tumors. Background technique [0002] Immunotherapy has become an emerging tumor therapy after surgery, radiotherapy, and chemotherapy, gradually showing its irreplaceable advantages. Tumor occurrence is usually accompanied by the exhaustion of the body's immune system. The purpose of immunotherapy is to reduce the exhaustion level of the body, reshape the immune system, and help the body eliminate tumor cells. The inflammatory microenvironment formed by inflammatory cells and mediators (cytokines, chemokines, etc.) is an important component of the tumor microenvironment. It is closely related to the immune surveillance and immune escape of tumors, and participates in the occurrence, development, invasion and metastasis of tumors. and other processes. A variety of inflamm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P35/00
CPCA61K38/164A61P35/00
Inventor 刘智杨祥良石林林
Owner 君维安(武汉)生命科技有限公司
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