Environmentally-friendly biological microbial inoculum for repairing soil pollution and preparation method of environmentally-friendly biological microbial inoculum for repairing soil pollution

A biological agent and soil restoration technology, applied in the field of soil restoration, can solve the problems of soil biological flora destruction, structural stickiness, and destruction of microbial flora structure, so as to increase the number of viable bacteria, improve water retention, and promote reproduction Effect

A biological agent and soil restoration technology, applied in the field of soil restoration, can solve the problems of soil biological flora destruction, structural stickiness, and destruction of microbial flora structure, so as to increase the number of viable bacteria, improve water retention, and promote reproduction Effect

CN110564648AActive Publication Date: 2019-12-13NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Environmentally-friendly biological microbial inoculum for repairing soil pollution and preparation method of environmentally-friendly biological microbial inoculum for repairing soil pollution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] An environmentally friendly biological agent for remediating soil pollution, mainly comprising the following raw material components in parts by weight: 40 parts of mixed chitosan gel, 8 parts of Acetobacter xylinum, 10 parts of Trichoderma and 12 parts of modified microspheres.

[0030] A method for preparing an environmentally friendly biological agent for repairing soil pollution, the method for preparing an environmentally friendly biological agent for repairing soil pollution mainly includes the following preparation steps:

[0031](1) Mix cholesterol and pyridine in a beaker at a mass ratio of 1:25, and add succinic anhydride 0.8 times the mass of cholesterol to the beaker, and stir for 6 hours at a temperature of 60°C and a speed of 300r / min. , the material in the beaker was concentrated by rotary evaporation for 5 hours at a temperature of 80°C, a rotational speed of 120r / min, and a pressure of 600kPa to obtain a crude product, which was recrystallized in an eth...

Embodiment 2

[0037] An environmentally friendly biological agent for remediating soil pollution, mainly comprising the following raw material components in parts by weight: 40 parts of chitosan gel, 8 parts of Acetobacter xylinum, 10 parts of Trichoderma and 12 parts by weight modified microspheres.

[0038] A method for preparing an environmentally friendly biological agent for repairing soil pollution, the method for preparing an environmentally friendly biological agent for repairing soil pollution mainly includes the following preparation steps:

[0039] (1) Mix cholesterol and pyridine in a beaker at a mass ratio of 1:25, and add succinic anhydride 0.8 times the mass of cholesterol to the beaker, and stir for 6 hours at a temperature of 60°C and a speed of 300r / min. , the material in the beaker was concentrated by rotary evaporation for 5 hours at a temperature of 80°C, a rotational speed of 120r / min, and a pressure of 600kPa to obtain a crude product, which was recrystallized in an e...

Embodiment 3

[0045] An environmentally friendly biological agent for remediating soil pollution, mainly comprising the following raw material components in parts by weight: 40 parts of mixed chitosan gel, 8 parts of Acetobacter xylinum, 10 parts of Trichoderma and 12 parts microspheres.

[0046] A method for preparing an environmentally friendly biological agent for repairing soil pollution, the method for preparing an environmentally friendly biological agent for repairing soil pollution mainly includes the following preparation steps:

[0047] (1) Mix cholesterol and pyridine in a beaker at a mass ratio of 1:25, and add succinic anhydride 0.8 times the mass of cholesterol to the beaker, and stir for 6 hours at a temperature of 60°C and a speed of 300r / min. , the material in the beaker was concentrated by rotary evaporation for 5 hours at a temperature of 80°C, a rotational speed of 120r / min, and a pressure of 600kPa to obtain a crude product, which was recrystallized in an ethanol soluti...

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Abstract

The invention discloses an environmentally-friendly biological microbial inoculum for repairing soil pollution and a preparation method of the environmentally-friendly biological microbial inoculum for repairing soil pollution, and belongs to the technical field of soil repairing. The preparation method comprises the steps of modifying glucan, coating 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimidehydrochloride and N-hydroxy succinimide with the modified glucan and polylactic acid so as to obtain modified microspheres; then mixing chitosan with agarose, and adding the modified microspheres soas to obtain semi-finished products; and finally, mixing the semi-finished products with a bacterial culture solution, adding trichoderma and acetobacter xylinum, performing mixed culture, and performing filtration so as to obtain the environmentally-friendly biological microbial inoculum for repairing soil pollution. After the prepared environmentally-friendly biological microbial inoculum for repairing soil pollution is used, the content of viable counts in soil can be effectively increased, and the content of effective state heavy metal ions in the soil is reduced.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to an environment-friendly biological bacterial agent for remediating soil pollution and a preparation method thereof. Background technique [0002] At present, according to the survey of relevant national departments, soil damage is mainly caused by pollution, while land pollution is mainly concentrated in heavy metal pollution, such as: cadmium, chromium, lead, mercury and other heavy metals, and cadmium pollution is more common. The soil is a complex of organic and inorganic combinations, which is the basis of agricultural planting, and organic fertility is the key factor for the quality assurance of agricultural production. Because the soil itself has a variety of organic and inorganic nutrients and microbial groups composed of a variety of microorganisms , provide a good growth environment for the growth of crops through the interaction of microbial populations and nut...

Claims

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Application Information

Patent Timeline
13 Dec 2019
Publication
CN110564648A
IPC
C12N1/20; C12N1/14; C12R1/02; C12R1/885; B09C1/10; B09C1/00
CPC
B09C1/10; B09C1/105; B09C2101/00; C12N11/10; C12N11/096
Inventors
吴昊