Electrode for micro-administration in vivo and conducting electrophysiological recording

Abstract

The invention discloses an electrode for micro-administration in vivo and conducting electrophysiological recording. The electrode includes an administration electrode, a recording electrode and a recording electrode sealer, a glass microelectrode is arranged on the lower segment of the administration electrode, the top end of the glass microelectrode is connected to the lower end of a silicone sleeve by a lower fixing ring of a metal right-angle adapter, the upper end of the silicone sleeve is connected to an administration soft hose by an upper fixing ring of the metal right-angle adapter, and a gap is formed between the umbrella part of the recording electrode and an opening of the tail end of the glass microelectrode. According to the electrode for micro-administration in vivo and conducting electrophysiological recording, the tip end of the recording electrode penetrates through the opening of the tail end of the glass microelectrode, the tip end of the glass microelectrode has asmall overall diameter, damage to the tissue is small, through micro-injection, precise administration of tiny nuclei can be achieved, the lengths of the glass microelectrode and the recording electrode can be adjusted according to the actual situation during making, and the electrode is suitable for administration and recording of an encephalic region with at different depths.

Description

technical field [0001] The invention relates to the related technical field of electrophysiological recording, in particular to an electrode for microdosing in vivo and performing electrophysiological recording. Background technique [0002] In biological and medical research, involving in extracellular electrophysiological recording (such as action potential, field potential, LTP / LTD, etc.), the common method is to fix the head of experimental animals (such as rats or mice) in a stereotaxic instrument Above, electrodes are inserted into the target brain region via the manipulator or micro-thrusters to record. If you want to study how a certain drug affects the activity of neurons in this brain area, some experiments inject drugs through the abdominal cavity, tail vein or lateral ventricle, and the drug effect often takes a long time, and the experimental results are inaccurate due to the wide range of drug effects; Implanting a microinjection needle into the position of th...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide an electrode for microdosing and electrophysiological recording in vivo, so as to solve the problem of inaccurate response of neurons to drug administration proposed in the above-mentioned background technology: such as injecting drugs through the abdominal cavity, tail vein or ventricle , the problem of long drug effect time and wide range, the problem of increasing the difficulty and accuracy of the experiment by administering the drug at the recording electrode position through the microinjection needle, the problem of increasing the cost or tissue damage through microelectrophoresis or multi-tube microelectrodes, etc.

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