Application of UB2/UB3 gene in regulation and control of corn multi-spike development

Abstract

The invention discloses application of a UB2 / UB3 gene in regulation and control of corn multi-spike development, and particularly relates to application of UB2 and UB3 gene double mutation in regulation and control of corn multi-spike development. According to the invention, gene editing is carried out on two targets UB2 and UB3 in corn by using a CRISPR / Cas9 transgenic technology, so that the target site of the UB2 gene has deletion of a basic group to cause frameshift mutation and deletion of large fragments of the UB3 gene. Results show that the tassel branch number of UB2 and UB3 double mutant plants is reduced, the increase of the spike number of the UB2 and UB3 double mutants is observed for the first time, new female spikes grow on spike stalks of main spikes, the multi-spike property is shown, auxiliary meristem at the lower part is also derepressed to be developed into female spikes, and phenotypes with increased aerial roots are accompanied; and it shows that after the UB2 gene and the UB3 gene are knocked out, a corn mutant plant can show a multi-spike phenotype. The invention provides a theoretical basis for genetic improvement of the corn multi-spike property has a large application prospect.

Description

technical field

[0001] The invention relates to the technical field of maize genetics and breeding, and more specifically relates to the application of UB2 / UB3 genes in regulating the development of multiple ears of maize. Background technique

[0002] Maize (Latin scientific name: Zea mays L.) is an annual herbaceous plant of the Poaceae Maize genus. Maize is an annual monoecious cross-pollinated plant with tall plants and strong stems. The highest crop, its planting area and total output are second only to rice and wheat. Reasonably increasing maize planting density is one of the effective ways to increase maize yield per unit area, but the light quality change (low R / FR ratio) brought about by dense planting will trigger a series of shade avoidance responses in plants: such as increased plant height and ear height, resistance Poor lodging ability, reduced leaf area, reduced branching, prolonged flowering interval between male and female flowers, decreased seed setting ra...

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