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Molecular marker method for predicting and identifying chicken abdominal fat weight

A molecular marker, chicken belly fat technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems that have not yet been discovered, and achieve the effects of high accuracy, simple operation and low cost

Active Publication Date: 2019-12-17
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no report on the selection of chicken abdominal fat weight using the g.1033G>A site genotype of TCF21 gene as a molecular marker

Method used

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  • Molecular marker method for predicting and identifying chicken abdominal fat weight
  • Molecular marker method for predicting and identifying chicken abdominal fat weight
  • Molecular marker method for predicting and identifying chicken abdominal fat weight

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Embodiment 1

[0031] Example 1. Molecular marker methods for predicting and identifying chicken belly fat weight.

[0032] The molecular marker method for predicting and identifying chicken belly fat weight described in this embodiment includes the following steps:

[0033] 1) According to the chicken TCF21 gene SNP locus g.1033G> A. Design primers TCF21-F and TCF21-R with the upstream and downstream 394bp sequence to obtain amplification primers;

[0034] 2) Extract chicken genomic DNA;

[0035] 3) Use the amplified primers obtained in step 1) to use the DNA extracted in step 2) as a template to perform PCR amplification of chicken genomic DNA to obtain amplified products;

[0036] 4) Use restriction enzyme HinP1I to digest the amplified product obtained in step 3) to obtain the digested product;

[0037] 5) Use an agarose gel to separate the digested product obtained in step 4) by electrophoresis, determine the genotype according to the result of electrophoresis separation, compare the abdominal fat...

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Abstract

The invention discloses a molecular marking method for predicting and identifying chicken abdominal fat weight, belongs to the technical field of molecular marking methods, and aims to solve the problem that the chicken abdominal fat weight cannot be judged from phenotype. The invention provides the molecular marker method for predicting and identifying chicken abdominal fat weight. The method specifically comprises the following steps: designing primers according to upstream and downstream 394bp sequences of a chicken TCF21 gene SNP locus g.1033G>A; carrying out PCR amplification on chicken genome DNA according to the obtained primers; then carrying out enzyme digestion on an amplification product by utilizing restriction endonuclease; and carrying out electrophoretic separation on an enzyme digestion product so as to obtain a marker genotype of the abdominal fat weight of a broiler. The method provided by the invention has the characteristics of simple operation, low cost and high accuracy, can be used for automatic detection, is an effective molecular marker breeding means, and can greatly accelerate the breeding process of chickens.

Description

Technical field [0001] The invention relates to a molecular marking method for predicting and identifying the weight of chicken belly fat, belonging to the technical field of molecular marking methods. Background technique [0002] Broilers are the most commonly raised poultry by humans. Since the 1950s, breeding companies have relied on phenotypic selection methods to select broiler growth speed and meat yield traits, which have significantly improved the performance of broiler chickens, but With the rapid growth of broiler chickens, some negative problems such as physiological maladjustment have emerged, such as excessive abdominal fat accumulation, ascites syndrome, leg growth and development diseases, reduced immunity of broilers, sudden death syndrome, etc., these problems Caused huge economic losses to broiler breeding enterprises. The proper amount of abdominal fat deposition in broilers is necessary for the body's physiology. However, excessive abdominal fat deposition c...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125C12Q2521/301Y02P60/87
Inventor 张慧于家强李辉杨莉莉曹志平栾鹏李玉茂
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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