Mother culture medium for facilitating growth of edible fungus mycelia

A kind of mother seed medium and mycelia growth technology, applied in the direction of cultivation, application, plant cultivation, etc., can solve the problems of increasing the cultivation cost of oyster mushrooms, poor antibacterial effect, and long time-consuming, etc., to meet the large-scale industrialization Production demand, good antibacterial effect, low cost effect

Pending Publication Date: 2019-12-27
INST OF PLANT NUTITUION & RESOURCE ENVIRONMENT HENAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These mediums need to purchase raw materials to boil and filter, which takes a long time. If you buy the configured dry powder medium, the price is extremely expensive, which increases the cost of Pleurotus ostreatus cultivation; and the bacteriostatic effect of these mediums is very poor, so boiling or When preparing the medium, it is often necessary to add antibacterial drugs to the medium to inhibit the germination or reproduction of miscellaneous bacteria such as mold or bacteria

Method used

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  • Mother culture medium for facilitating growth of edible fungus mycelia
  • Mother culture medium for facilitating growth of edible fungus mycelia
  • Mother culture medium for facilitating growth of edible fungus mycelia

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1 The liquid parent medium for promoting the growth of edible fungus mycelia according to the present invention is prepared from corncobs, bran, urea and lime according to the following proportions by weight and methods:

[0029] Proportion: 80 parts of corn cob, 10 parts of bran, 1 part of urea, 5 parts of lime, and the ratio of material to water is 1:2.5;

[0030] The preparation method comprises the following steps:

[0031] The first step is to accurately weigh the materials according to the above ratio, the total weight of the four dry materials is 500kg, and uniformly mix the materials according to the material-to-water ratio (weight ratio) of 1:2.5 to obtain the mixture;

[0032] In the second step, use the mixture obtained in the first step to build a fermentation stockpile. The stockpile has a width of 1 meter, a height of 0.6 meters, and any length; Holes are drilled at intervals, and the distance between two adjacent holes is 30-35cm to ensure the...

Embodiment 2

[0041] Example 2 The dry powder mother seed culture medium for promoting the growth of edible fungus mycelia according to the present invention is prepared from raw materials corn cob, bran, urea and lime according to the following proportions by weight and method:

[0042] Proportion: 80 parts of corn cob, 10 parts of bran, 1 part of urea, 5 parts of lime, and the ratio of material to water is 1:2.5;

[0043] The preparation method comprises the following steps:

[0044] The first step and second step are all identical with embodiment 1;

[0045] The third step, after the fermentation is over, open the stockpile and cool it down to room temperature naturally, dry it at 50-60°C until the moisture in the fermentation mixture is ≤10%, crush it, and sieve it (300 mesh sieve) to get dry powder mother Seed culture medium.

[0046] The dry powder mother seed culture medium obtained by the present invention is dissolved in water, an appropriate amount of agar is added, and after be...

Embodiment 3

[0047] Embodiment 3 The bacteriostasis of mother seed culture medium of the present invention to mold

[0048] Add an appropriate amount of agar to the extract obtained in Example 1 (concentration is 300g / L), obtain the extract medium after high-temperature sterilization, pour the extract medium into a petri dish, inoculate Trichoderma, Mucor and Penicillium are three kinds of molds, each mold is inoculated with 10 dishes;

[0049] Using PDA medium as a control, pour the sterilized PDA medium into a petri dish, inoculate Trichoderma, Mucor, and Penicillium respectively, and inoculate 10 dishes with each type of mold;

[0050] All culture dishes were placed in an incubator at 28° C. for 3 days, and the mycelial growth and growth rate of each mold were observed. from figure 1 It can be seen that, compared with the PDA medium, the mother seed medium of the present invention can significantly inhibit the growth of Trichoderma, Mucor and Penicillium three kinds of mold hyphae, an...

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Abstract

The invention discloses a mother culture medium for facilitating growth of edible fungus mycelia. The mother culture medium comprises, by weight, 80 parts of corncobs, 10 parts of bran, 1 part of ureaand 5 parts of lime, and the ratio of material to water is 1:2.5. A preparation method of the mother culture medium includes the steps: firstly, accurately weighing the materials according to the ratio, and uniformly mixing the materials according to the material and water ratio of 1:2.5 to obtain mixture; secondly, building a fermentation material pile with the mixture acquired in the first step, and holing from the top to the bottom at intervals; thirdly, turning the fermentation material pile over, cooling, drying and crushing fermentation materials after mixture is completely fermented, and screening the crushed mixture to obtain dry mother culture medium. According to the mother culture medium, raw materials are low in cost and easily acquired, the mother culture medium has great significance for great improvement of resource utilization rate, mushroom production rate and labor productivity rate, a good production mode is provided for agricultural green development, and the mother culture medium is good in antibacterial effect and applicable to culture of various edible fungi.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation, in particular to a mother seed culture medium for promoting the growth of edible fungus hyphae. Background technique [0002] Edible fungi are large edible fungi. There are more than 350 kinds of edible fungi known in China. At present, edible fungi such as Pleurotus eryngii, Pleurotus eryngii, and fungus have become must-have dishes on the public table due to their rich nutrition and moderate price, and have been widely cultivated around the world, such as China, Japan, India, Germany, South Korea, France and other major producing countries. [0003] In the cultivation technology of edible fungi, since the mother species of edible fungi (such as oyster mushroom, fungus, etc.) After the seed is transferred and expanded, it is inoculated into the bacteria bag or inoculation bottle. In the transfer expansion culture, the growth and growth speed of the mother species of mycelia directly a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20
CPCA01G18/20
Inventor 孔维丽刘芹崔筱张玉亭袁瑞奇康源春孔维威段亚魁胡素娟宋志波徐柯韩玉娥
Owner INST OF PLANT NUTITUION & RESOURCE ENVIRONMENT HENAN ACADEMY OF AGRI SCI
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